• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. • Sensitivity: The limit of detection of this assay is 0.5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 93/722. • Recovery: A mid-curve recovery of 76 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 2.5% (CV). The inter-assay precision of this assay is 9.8% (CV).
*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
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