人GM-CSF (CSF2)ELISA试剂盒

用于人粒细胞-巨噬细胞集落刺激因子的检测和测定

产品号 #(选择产品)

产品号 #100-1146_C

用于人粒细胞-巨噬细胞集落刺激因子的检测和测定

产品组分包括

  • 人GM-CSF (CSF2)ELISA试剂盒
    • 人GM-CSF ELISA酶标板,1x
    • 人GM-CSF HRP检测抗体,1瓶
    • 人GM-CSF标准品,1瓶
    • 洗涤缓冲液(20X), 30mL
    • 稀释缓冲液(20X), 12.5 mL
    • 过氧化物溶液,12.5 mL
    • TMB底物,12.5 mL
    • 终止液,11mL

总览

人GM-CSF ELISA试剂盒用于定量检测细胞培养上清液中的粒细胞-巨噬细胞集落刺激因子 (GM-CSF),也称为CSF2。GM-CSF 由活化的 T 细胞、B 细胞、巨噬细胞、单核细胞、内皮细胞和成纤维细胞在促炎刺激后分泌。GM-CSF 受体 CSF2R 由一条 α 链和一条 β 链组成。β 链与白细胞介素 3 (IL-3) 和 5 (IL-5) 受体共享,与 GM-CSF 具有低亲和力和高亲和力结合。GM-CSF 的活性形式以同型二聚体形式存在,编码基因位于 5q31 染色体区域的一个基因簇中,该区域因 5q 减 (5q-) 综合征和髓性白血病而闻名。 GM-CSF 的过度表达可能是炎症和自身免疫性疾病(例如关节炎、肾炎和牛皮癣)的关键指标。

该检测基于夹心 ELISA 方法,将样品加入预先包被有针对人 GM-CSF 特异性捕获抗体的 ELISA 可拆酶标板。通过添加辣根过氧化物酶 (HRP) 偶联的检测抗体来检测捕获的 GM-CSF。添加显色酶底物 3,3'5,5' 四甲基联苯胺 (TMB) 后,会产生有色产物,其强度与样品中人 GM-CSF 的浓度成正比。

亚型
完整试剂盒
 
细胞类型
B 细胞,内皮细胞,成纤维细胞,巨噬细胞,单核细胞,T 细胞
 
种属

 
应用
ELISAs
 
研究领域
免疫,感染性疾病(传染病)
 

Data Figures

Representative Standard Curve Of Human IL-22

Figure 1. Representative Standard Curve Of Human GM-CSF (CSF2)

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 6.25 - 400 pg/mL. The lower limit of detection (LLD) of this assay is 5.43 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 98 - 103% was determined by spiking defined amounts of analyte standard into cell culture supernatant in repeated experiments. The intra-assay precision of this assay is 4.4% (CV). The Inter-assay precision of this assay is 1.8% (CV).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Safety Data Sheet 1
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Safety Data Sheet 8
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Species Human
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