Perform gentle enzymatic dissociation of mouse mammary tissue with 10X Collagenase/Hyaluronidase in Dulbecco’s Modified Eagle’s Medium (DMEM). This gentle formulation enables overnight dissociation of mouse mammary gland tissue so experiments can be completed the next day rather than late at night. Gentle Collagenase/Hyluronidase has also been used for enzymatic dissociation of mouse prostate epithelial cells (Frégeau-Proulx et al. MethodsX, 2022). This protease/polysaccharidase combination digests native collagen fibrils and hydrolizes hyaluronic acids in connective tissues for effective tissue dissociation.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Gentle Collagenase/Hyaluronidase
Catalog #
07919
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Gentle Collagenase/Hyaluronidase
Catalog #
07919
Lot #
All
Language
English
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