EasySep™ Release 小鼠APC正选试剂盒

使用磁珠解离技术的免疫磁珠正选试剂盒

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产品号 #100-0033

通过免疫磁珠正选，分选出APC偶联抗体标记但无磁珠标记的小鼠细胞

产品优势

在40分钟内从小鼠组织中分离出用APC偶联抗体标记的高纯度细胞
无需清洗去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

EasySep™ Release 小鼠APC正选试剂盒（产品号 #100-0033）
- EasySep™ Release APC正选抗体混合物，1 mL
- EasySep™ Releasable RapidSpheres™，1 mL
- EasySep™Release 缓冲液（浓缩），3 x 1 mL
- 用于正选的抗小鼠FcR阻断剂，0.5 mL

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EasySep™ Release 小鼠APC正选试剂盒

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实验数据

$Using the EasySep™ Release Mouse APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.$

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

产品说明书及文档

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Publications (1)

Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging. A. K. Jaiswal et al. American journal of physiology. Lung cellular and molecular physiology 2022 jan

Abstract

Asthma and its heterogeneity change with age. Increased airspace neutrophil numbers contribute to severe steroid-resistant asthma exacerbation in the elderly, which correlates with the changes seen in adults with asthma. However, whether that resembles the same disease mechanism and pathophysiology in aged and adults is poorly understood. Here, we sought to address the underlying molecular mechanism of steroid-resistant airway inflammation development and response to corticosteroid (Dex) therapy in aged mice. To study the changes in inflammatory mechanism, we used a clinically relevant treatment model of house-dust mite (HDM)-induced allergic asthma and investigated lung adaptive immune response in adult (20-22 wk old) and aged (80-82 wk old) mice. Our result indicates an age-dependent increase in airway hyperresponsiveness (AHR), mixed granulomatous airway inflammation comprising eosinophils and neutrophils, and Th1/Th17 immune response with progressive decrease in frequencies and numbers of HDM-bearing dendritic cells (DC) accumulation in the draining lymph node (DLn) of aged mice as compared with adult mice. RNA-Seq experiments of the aged lung revealed short palate, lung, and nasal epithelial clone 1 (SPLUNC1) as one of the steroid-responsive genes, which progressively declined with age and further by HDM-induced inflammation. Moreover, we found increased glycolytic reprogramming, maturation/activation of DCs, the proliferation of OT-II cells, and Th2 cytokine secretion with recombinant SPLUNC1 (rSPLUNC1) treatment. Our results indicate a novel immunomodulatory role of SPLUNC1 regulating metabolic adaptation/maturation of DC. An age-dependent decline in the SPLUNC1 level may be involved in developing steroid-resistant airway inflammation and asthma heterogeneity.

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EasySep™ Release 小鼠APC正选试剂盒

产品号 #100-0033

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EasySep™ Release 小鼠APC正选试剂盒

产品号 #100-0033

产品优势

产品组分包括

概述

实验数据

产品说明书及文档

相关材料与文献

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