EasySep™发布人APC阳性选择试剂盒

采用颗粒释放技术的免疫磁阳性选择试剂盒

产品号 #(选择产品)

产品号 #100-0031_C

采用颗粒释放技术的免疫磁阳性选择试剂盒

产品优势

  • 用apc偶联抗体标记的高纯度细胞在不到40分钟内从人样品中分离出来
  • EasySep™releable RapidSpheres™免洗去除

产品组分包括

  • EasySep™发布人类APC阳性选择试剂盒(目录#100-0031)
    • EasySep™Release APC阳性选择鸡尾酒,1ml
    • EasySep™可释放RapidSpheres™,1ml
    • EasySep™释放缓冲液(浓缩),3 × 1ml
    • 抗人CD32阳性选择阻断剂,1ml

概述

EasySep™Release Human APC阳性选择试剂盒旨在通过免疫磁阳性选择从新鲜或先前冷冻的外周血单个核细胞或洗净的白细胞取样中分离带有APC偶联抗体的细胞。

用抗体和磁性颗粒标记所需细胞,并使用EasySep™磁铁无柱分离。不需要的细胞被简单地倒出,而需要的细胞留在管中。然后,从EasySep™分离的apc抗体标记的细胞中去除结合的磁性颗粒,这些细胞立即可用于下游应用。使用EasySep™释放试剂盒分离细胞后,抗体复合物仍与细胞表面结合,并可能与Brilliant Violet™抗体偶联物、聚乙二醇修饰蛋白或其他化学相关配体相互作用。

Magnet Compatibility

• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
 
Subtype
Cell Isolation Kits
 
Cell Type
Other
 
Species
Human
 
Sample Source
Leukapheresis, Other, PBMC
 
Selection Method
Positive
 
Application
Cell Isolation
 
Brand
EasySep
 
Area of Interest
Immunology
 

Data Figures

Using the EasySep™ Release Human APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 5.9% and 94.2%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Human APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 5.9% and 94.2%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0031
Lot #
1000157896 or lower
Language
English
Catalog #
100-0031
Lot #
1000157897 or higher
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0031
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
100-0031
Lot #
All
Language
English

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102)
Sample Source Leukapheresis, Other, PBMC
Selection Method Positive
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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