产品号 #17879_C
免疫磁珠正选试剂盒
免疫磁珠正选试剂盒
EasySep™ 人全血CD34正选试剂盒II为从人新鲜全血或新鲜制备的白膜层中正选CD34+细胞而设计。目的细胞被CD34的抗体四聚体复合物和磁珠标记。同时,抗体混合物中还含有人Fc受体抗体,可减少非特异性结合。使用EasySep™磁极分选被标记的细胞,无需使用分离柱。目的细胞被保留在试管中,而非目的细胞被倾倒出。CD34抗原在造血干细胞和祖细胞上表达。
本产品可替代EasySep™ 人全血CD34正选试剂盒(产品号 #18086)。
如需从新鲜脐带血中分选CD34+细胞,建议使用EasySep™人脐带血CD34正选试剂盒II(产品号 #17896)。 如需从其它类型样本中分选CD34+细胞,请使用EasySep™人CD34正选试剂盒II(产品号 #17856)
磁体兼容性
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
亚型
细胞分选试剂盒
细胞类型
造血干/祖细胞
种属
人
样本来源
Buffy Coat,Whole Blood
筛选方法
Positive
应用
细胞分选
品牌
EasySep,RoboSep
研究领域
嵌合体,免疫,干细胞生物学
Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using RoboSep™-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human |
---|---|
Magnet Compatibility | • "The Big Easy" EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000) |
Sample Source | Buffy Coat, Whole Blood |
Selection Method | Positive |
细胞分选缓冲液
全自动细胞分选仪器
小鼠单克隆IgG1抗体,抗人CD34
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