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EasySep™人PE正选试剂盒II

免疫磁珠正选
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产品号 #（选择产品）

产品号 #17664_C

免疫磁珠正选

产品优势

  • 快速、简单
  • 无需分离柱

产品组分包括

  • EasySep™人PE正选试剂盒II（产品号 ＃17664）
    • EasySep™PE分选抗体混合物，1 mL
    • 抗人CD32 FcR阻断剂，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 一抗偶联物专用管（手动操作无需使用），1支
  • EasySep™人PE正选试剂盒II（产品号 ＃17694）
    • EasySep™人PE正选试剂盒II（产品号 ＃17664）x 5
  • RoboSep™ 人PE正选试剂盒II（产品号 ＃17664RF）
    • EasySep™PE分选抗体混合物，1 mL
    • 抗人CD32 FcR阻断剂，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 一抗偶联物专用管（手动操作无需使用），1支
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号 #20125）x 2
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
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产品说明书及文档
相关材料与文献
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总览

EasySep™人PE正选试剂盒II旨在通过正选分离PE（藻红蛋白）偶联抗体标记的细胞。目的细胞被识别PE的抗体复合物和葡聚糖包被的磁珠标记。通过EasySep™磁极分离被标记的细胞，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。

本产品可替代EasySep™人PE正选试剂盒（产品号 #17654）。

如需高纯度无磁珠标记的细胞，我们推荐使用EasySep™ Release人PE正选试剂盒（产品号#17654）。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
种属
人
 
样本来源
Buffy Coat，Cord Blood，Leukapheresis，其它细胞系，PBMC
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Data Figures

Starting with human PBMCs, the purities of the start and final isolated fractions in the above example are 59.1% and 98.7%, respectively, using a PE-conjugated anti-human CD3 antibody and EasySep™ Human PE Positive Selection Kit II.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17694, 17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Human PE Positive Selection Kit II
Catalog #
17664RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17694, 17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17694, 17664
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human PE Positive Selection Kit II
Catalog #
17664
Lot #
All
Language
English

Resources and Publications

Educational Materials (8)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
How to Isolate Antigen-Specific T Cells
Protocol
How to Isolate Antigen-Specific T Cells
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Publications (2)

Clostridium perfringens $\alpha$-toxin up-regulates plasma membrane CD11b expression on murine neutrophils by changing intracellular localization. M. Takehara et al. Biochimica et biophysica acta. Biomembranes 2022 dec

Abstract

Gas gangrene caused by Clostridium perfringens type A infection is a highly lethal infection of soft tissue characterized by rapid spread of tissue necrosis. This tissue destruction is related to profound attenuation of blood flow accompanied by formation of platelet-leukocyte aggregates in the blood vessels. Several studies have identified $\alpha$-toxin, which has both sphingomyelinase and phospholipase C activities, as a major virulence factor in the aggregate formation via activation of the platelet gpIIbIIIa. Here, we show that $\alpha$-toxin greatly and rapidly increases plasma membrane localization of CD11b, which binds to the platelet gpIIbIIIa via fibrinogen, in mouse neutrophils. Interestingly, short-term treatment of $\alpha$-toxin has little effect on gene expression profiles in neutrophils, and the toxin does not change the total protein expression levels of CD11b in whole cell lysates. The following analysis demonstrated that CD11b localizes to intracellular vesicles in intact cells, but the localization changed to the cytoplasmic membrane in $\alpha$-toxin-treated cells. These results suggest that CD11b is recruited to the cytoplasmic membrane by $\alpha$-toxin. Previously, we reported that $\alpha$-toxin promotes the formation of ceramide by its sphingomyelinase activity in mouse neutrophils. Interestingly, a synthetic cell-permeable ceramide analog, C2-ceramide, increases plasma membrane localization of CD11b, suggesting that ceramide production by $\alpha$-toxin recruits CD11b to the cytoplasmic membrane to promote platelet-leukocyte aggregation. Together, our results illustrate that the increase of cell membrane CD11b expression by $\alpha$-toxin might be crucial for the pathogenesis of C. perfringens to promote formation of platelet-leukocyte aggregates, leading to rapid tissue necrosis due to ischemia.
View publication
Endothelin-A Receptor Antagonist Alleviates Allergic Airway Inflammation via the Inhibition of ILC2 Function. X. Zhang et al. Frontiers in immunology 2022

Abstract

Allergic airway inflammation is a universal airway disease that is driven by hyperresponsiveness to inhaled allergens. Group 2 innate lymphoid cells (ILC2s) produce copious amounts of type 2 cytokines, which lead to allergic airway inflammation. Here, we discovered that both peripheral blood of human and mouse lung ILC2s express the endothelin-A receptor (ETAR), and the expression level of ETAR was dramatically induced upon interleukin-33 (IL-33) treatment. Subsequently, both preventive and therapeutic effects of BQ123, an ETAR antagonist, on allergic airway inflammation were observed, which were associated with decreased proliferation and type 2 cytokine productions by ILC2s. Furthermore, ILC2s from BQ123 treatment were found to be functionally impaired in response to an interleukin IL-33 challenged. And BQ123 treatment also affected the phosphorylation level of the extracellular signal-regulated kinase (ERK), as well as the level of GATA binding protein 3 (GATA3) in activated ILC2s. Interestingly, after BQ123 treatment, both mouse and human ILC2s in vitro exhibited decreased function and downregulation of ERK signaling and GATA3 stability. These observations imply that ETAR is an important regulator of ILC2 function and may be involved in ILC2-driven pulmonary inflammation. Therefore, blocking ETAR may be a promising therapeutic strategy for allergic airway inflammation.
View publication
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更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Buffy Coat, Cord Blood, Leukapheresis, Other, PBMC
Selection Method Positive
Legal Statement: Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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