产品号 #100-1131
人EGFR+细胞免疫磁珠正选
使用EasySep™人EGFR正选试剂盒,可在短至15分钟内从新鲜或冻存的人外周血单个核细胞(PBMCs)、白细胞单采或细胞培养样本中高效分选表达表皮生长因子受体(EGFR)的细胞。分选后的细胞可立即用于下游应用,例如流式细胞术、培养或DNA/RNA 提取。使用免疫磁珠EasySep™技术,EGFR+细胞通过抗体和磁珠标记,无需分选柱即可实现分选。标记的细胞保留在试管中,而未被标记的细胞可被倾倒出。根据您的研究目的,未被标记的细胞也
可用于下游应用。本试剂盒可识别 EGFR,该受体属于ErbB家族酪氨酸激酶受体(RTK),表达于循环肿瘤细胞(CTCs)、巨噬细胞、单核细胞、浆细胞及某些T细胞亚群(如效应CD4+ T细胞和表达FoxP3的调节性CD4+ T细胞(Tregs)表面 (Sabbah DA et al. Curr Top Med Chem, 2020 and MacDonald F& Zaiss DMW Front Pharmacol, 2017)。深入了解免疫磁珠EasySep™技术原理,探索我们的原代与培养的细胞产品。
MAGNET COMPATIBILITY
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
SUBTYPE
Cell Isolation Kits
CELL TYPE
Brain Tumor Stem Cells, Cancer Cells and Cell Lines, Macrophages, Monocytes, Mononuclear Cells, T Cells, T Cells, CD4+, Tumor Cells
SPECIES
Human
SAMPLE SOURCE
Leukapheresis, PBMC
SELECTION METHOD
Positive
BRAND
EasySep
AREA OF INTEREST
Cancer, Immunology, Cell Therapy Development
Figure 1. Typical EasySep™ Human EGFR Positive Selection Kit Isolation Profile
Starting with 10% A549 cells spiked into human PBMCs, the EGFR+ cell content of the isolated fraction is typically 95.0 ± 2.8% (mean ± SD using the purple EasySep™ Magnet). In the above example, the frequencies of the start (left) and final (right) isolated fractions are 10.3% and 95.0%, respectively.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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