EasySep™人CD4+ CD127low CD49d-调节性T细胞富集试剂盒

免疫磁珠负选试剂盒

产品号 #(选择产品)

产品号 #19232_C

免疫磁珠负选试剂盒

产品优势

  • 操作简单、快捷,且无需分离柱
  • 纯度高达87.4%
  • 分选得到的细胞不带标记     

产品组分包括

  • EasySep™人CD4+  CD127low CD49d-调节性T细胞富集试剂盒(产品号 #19232)
    • EasySep™人CD4+  CD127low CD49d-调节性T细胞富集抗体混合物,1 mL
    • EasySep™  葡聚糖分选抗体混合物,1 mL
    • EasySep™ Magnetic Particles磁珠,3 x 1 mL
  • RoboSep™ CD4+CD127lowCD49d-  调节性T细胞富集试剂盒(产品号 #19232RF)
    • EasySep™人CD4+ CD127low CD49d- 调节性T细胞富集抗体混合物,1 mL
    • EasySep™葡聚糖分选抗体混合物,1 mL
    • EasySep™ Magnetic Particles磁珠 ,3 x 1 mL
    • RoboSep™ 缓冲液(产品号 #20104)
    • RoboSep™过滤吸头(产品 号 #20125) 
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总览

EasySep™  人CD4+CD127lowCD49d- 调节性T细胞富集试剂盒专为通过免疫磁珠负选的方式 从新鲜外周血单个核细胞中分离CD4+CD127lowCD49d-细胞而设计。利用识别非 CD4+ T细胞、CD127high和CD49d+细胞的抗体四聚体复合物以及葡聚糖包被的磁珠,靶向去除非目的细胞。通过EasySep™磁极分离被标记的细胞,无需使用分离柱,目的细胞直接倾倒至新的管中即可。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞,T 细胞,CD4+,T 细胞,调节性细胞
 
种属

 
样本来源
PBMC
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep,RoboSep
 
研究领域
免疫
 

Data Figures

Typical EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Figure 1. Typical EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Profile

Starting with fresh PBMCs, the CD3+CD4+CD127lowCD49d-CD25+FOXP3+ cell content of the enriched fraction typically ranges from 57.4 - 87.4%. Donor to donor variability is unavoidable and may affect the purity of the enriched fraction. In the example above, the purities of the start and enriched fractions are 1.84% and 84.1%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 3
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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 4
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19232RF
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Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (9)

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Publications (1)

A Transendocytosis Model of CTLA-4 Function Predicts Its Suppressive Behavior on Regulatory T Cells Hou TZ et al. The Journal of Immunology 2015

Abstract

Manipulation of the CD28/CTLA-4 pathway is at the heart of a number of immunomodulatory approaches used in both autoimmunity and cancer. Although it is clear that CTLA-4 is a critical regulator of T cell responses, the immunological contexts in which CTLA-4 controls immune responses are not well defined. In this study, we show that whereas CD80/CD86-dependent activation of resting human T cells caused extensive T cell proliferation and robust CTLA-4 expression, in this context CTLA-4 blocking Abs had no impact on the response. In contrast, in settings where CTLA-4(+) cells were present as regulators�

更多信息

更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
Sample Source PBMC
Selection Method Negative
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