产品号 #17874_C
从全血中免疫磁珠正选分选人CD19+细胞
从全血中免疫磁珠正选分选人CD19+细胞
使用EasySep™ HLA嵌合全血CD19正选试剂盒,通过免疫磁珠正选技术,从全血、白膜层或LRS去白系统(LRSC;LRS cone)样本中分离高纯度人CD19+细胞。EasySep™技术结合单克隆抗体的特异性和无需分离柱的简便磁分选系统,已在发表的研究中广泛应用超过20年。
在该EasySep™阳性分选流程中,目的细胞通过与识别CD19的抗体复合物及磁珠进行标记。抗体复合物中还含有人Fc受体的结合抗体,可最大程度地减少非特异性结合。使用EasySep™磁分选系统分离标记细胞,只需倾倒或移液吸取非目的细胞。目的细胞保留在分离管中。经磁珠分选后,目的CD19+细胞即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。CD19抗原存在于成熟B细胞和B祖细胞表面,但在分化成浆细胞时丢失。该抗原亦在滤泡树突状细胞上表达。
该产品可替代EasySep™人全血CD19细胞正选试剂盒 (产品号 #18084) 可实现 更快地细胞分选。
深入了解免疫磁珠EasySep™技术的工作原理,或如何通过RoboSep™实现免疫磁珠细胞分选全自动化以节省时间并提升实验室通量。探索更多为您的实验流程优化的产品,包括细胞表征、冷冻保存等相关产品。
磁体兼容性
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
亚型
细胞分选试剂盒
细胞类型
B 细胞
种属
人
样本来源
Buffy Coat,Whole Blood
筛选方法
Positive
品牌
EasySep
研究领域
HLA,免疫
Figure 1. Typical EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Profile
Starting with human whole blood, the CD19+ cell content of the isolated fraction typically ranges from 94.3 - 99.6% (as assessed by staining the start and isolated fractions with anti-CD19 or anti-CD20 antibodies, respectively). In the above example, the purities of the start and the final isolated fractions are 5.0% and 98.6%, respectively (gated on CD45).
NOTE: Red blood cells were removed from the start sample by lysis prior to flow cytometry.
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Human |
---|---|
Magnet Compatibility | • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000) |
Sample Source | Buffy Coat, Whole Blood |
Selection Method | Positive |
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