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EasySep™ HLA嵌合全血CD15正选试剂盒

免疫磁珠正性分选细胞分选试剂盒
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产品号 #（选择产品）

产品号 #17881_C

免疫磁珠正性分选细胞分选试剂盒

产品优势

  • 操作简单、快速
  • 纯度高达99%
  • 无需分离柱

产品组分包括

  • EasySep™ HLA嵌合全血CD15正选试剂盒（产品号 #17881）    
    • EasySep™ HLA嵌合全血CD15正选抗体混合物，3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102磁珠，3 x 1 mL
    • EasySep™10X浓缩红细胞裂解试剂，10 mL（产品号#20110）
  • RoboSep™ HLA嵌合全血CD15正选试剂盒（含过滤枪头）（产品号17881RF）
    • EasySep™人全血CD15正选抗体混合物，3 x 1 mL    
    • EasySep™ Dextran RapidSpheres™ 50102磁珠，3 x 1 mL
    • EasySep™ 10X浓缩红细胞裂解试剂，10 mL（产品号#20110）    
    • RoboSep™ 缓冲液（产品号 #20104）x 2
    • RoboSep™ 过滤枪头（产品号20125）x 2
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    “The Big Easy”EasySep™磁铁
  2. EasySep™缓冲液
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

EasySep™ HLA嵌合全血CD15正选选试剂盒为从新鲜全血中正选高纯度CD15+细胞而设计。目的细胞被识别CD15的四聚体抗体复合物和磁珠标记，并通过 EasySep™ 磁极进行无柱分选。非目的细胞通过简单倾倒弃去， 而目的细胞则保留在试管中。CD15抗原在粒细胞和单核细胞上表达。

本产品可替代EasySep™人全血CD15正选试剂盒（产品号 #18681）和EasySep™ HLA全血CD15阳性分选试剂盒（产品号18681HLA），实现更快速的细胞分选     。

磁体兼容性
• “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
髓系细胞
 
种属
人
 
样本来源
PMNC，Whole Blood
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
嵌合体，HLA，免疫
 

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Data Figures

Starting with human whole blood, the CD15+ cell content of the isolated fraction is typically 99.2 ± 1.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 57.6% and 99.9%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Catalog #
17881
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Magnet Compatibility • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source PMNC, Whole Blood
Selection Method Positive
标记抗体
  1. 抗人CD14抗体，克隆M5E2
    抗人CD14抗体，克隆M5E2

    抗人、恒河猴、食蟹猴CD14的小鼠单克隆IgG2a抗体

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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