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EasySep™FITC正选试剂盒II

免疫磁珠细胞正选试剂盒
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产品号 #（选择产品）

产品号 #17682_C

免疫磁珠细胞正选试剂盒

产品优势

  • 快速、简单
  • 无需分离柱

产品组分包括

  • EasySep™FITC正选试剂盒II（产品号 ＃17682）
    • EasySep™FITC分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
  • RoboSep™ FITC正选试剂盒II（产品号 ＃17681RF）
    • EasySep™ FITC分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号#20125）x 2
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
产品说明书及文档
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总览

EasySep™FITC正选试剂盒II可通过正选方式将FITC偶联抗体结合的细胞分离出来。目的细胞被识别FITC和葡聚糖包被的磁珠的抗体复合物标记。标记细胞通过EasySep™磁极被分选，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。

本产品替代EasySep™FITC正选试剂盒（产品号#18558）以进行更快地细胞分选。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
种属
非人灵长类，其它细胞系，大鼠
 
样本来源
Bone Marrow，Buffy Coat，Cord Blood，Leukapheresis，其它细胞系，PBMC，外周血，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ FITC Positive Selection Kit II
Catalog #
17682
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ FITC Positive Selection Kit II
Catalog #
17682RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ FITC Positive Selection Kit II
Catalog #
17682
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ FITC Positive Selection Kit II
Catalog #
17682
Lot #
All
Language
English

Resources and Publications

Educational Materials (6)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Publications (1)

Innate lymphoid cells: NK and cytotoxic ILC3 subsets infiltrate metastatic breast cancer lymph nodes. L. Rethacker et al. Oncoimmunology 2022

Abstract

Innate lymphoid cells (ILCs) - which include cytotoxic Natural Killer (NK) cells and helper-type ILC - are important regulators of tissue immune homeostasis, with possible roles in tumor surveillance. We analyzed ILC and their functionality in human lymph nodes (LN). In LN, NK cells and ILC3 were the prominent subpopulations. Among the ILC3s, we identified a CD56+/ILC3 subset with a phenotype close to ILC3 but also expressing cytotoxicity genes shared with NK. In tumor-draining LNs (TD-LNs) and tumor samples from breast cancer (BC) patients, NK cells were prominent, and proportions of ILC3 subsets were low. In tumors and TD-LN, NK cells display reduced levels of NCR (Natural cytotoxicity receptors), despite high transcript levels and included a small subset CD127- CD56- NK cells with reduced function. Activated by cytokines CD56+/ILC3 cells from donor and patients LN acquired cytotoxic capacity and produced IFNg. In TD-LN, all cytokine activated ILC populations produced TNF$\alpha$ in response to BC cell line. Analyses of cytotoxic and helper ILC indicate a switch toward NK cells in TD-LN. The local tumor microenvironment inhibited NK cell functions through downregulation of NCR, but cytokine stimulation restored their functionality.
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更多信息

更多信息
Species Non-Human Primate, Other, Rat
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Bone Marrow, Buffy Coat, Cord Blood, Leukapheresis, Other, PBMC, Peripheral Blood, Spleen
Selection Method Positive
Legal Statement: Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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