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EasySep™Biotin正选试剂盒

生物素抗体标记细胞的免疫磁珠细胞正选试剂盒
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产品号 #（选择产品）

产品号 #17683_C

生物素抗体标记细胞的免疫磁珠细胞正选试剂盒

产品优势

  • 快速、简单
  • 无需分离柱

产品组分包括

  • EasySep™Biotin正选试剂盒II（产品号＃17683）
    • EasySep™ Biotin分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
  • RoboSep™Biotin正选试剂盒II（产品号 ＃17683RF）
    • EasySep™Biotin分选抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 管，用于一抗偶联抗体（手动使用时无需此项），1 vial
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤吸头（产品号#20125）x 2
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
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总览

使用EasySep™生物素正选试剂盒 II，通过免疫磁珠正选技术从任何单细胞悬液样本中分离出经生物素化抗体标记的高纯度细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

该EasySep™ 正选流程中，目的细胞被识别生物素和葡聚糖包被的磁珠的抗体复合物标记。标记细胞通过EasySep™磁极被分选，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。完成磁性细胞分选后，目标生物素阳性细胞即可用于流式细胞术、细胞培养或细胞实验等下游应用。

本产品替代EasySep™Biotin正选试剂盒（产品号#17683）以进行更快地细胞分选。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选实现全自动化。探索为您的实验流程优化的更多产品，包括培养基、添加剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
种属
非人灵长类，其它细胞系，大鼠
 
样本来源
Bone Marrow，Buffy Coat，Cord Blood，Leukapheresis，其它细胞系，PBMC，外周血，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Biotin Positive Selection Kit II
Catalog #
17683
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Biotin Positive Selection Kit II
Catalog #
17683RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Biotin Positive Selection Kit II
Catalog #
17683
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Biotin Positive Selection Kit II
Catalog #
17683
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Biotin Positive Selection Kit II
Catalog #
17683RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Biotin Positive Selection Kit II
Catalog #
17683RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Biotin Positive Selection Kit II
Catalog #
17683RF
Lot #
All
Language
English

Resources and Publications

Educational Materials (6)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep...
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Publications (1)

Thymocyte Maturation and Emigration in Adult Mice. K. Joannou et al. Journal of immunology (Baltimore, Md. : 1950) 2022 may

Abstract

Several unique waves of ?? T cells are generated solely in the fetal/neonatal thymus, whereas additional ?? T cell subsets are generated in adults. One intriguing feature of ?? T cell development is the coordination of differentiation and acquisition of effector function within the fetal thymus; however, it is less clear whether this paradigm holds true in adult animals. In this study, we investigated the relationship between maturation and thymic export of adult-derived ?? thymocytes in mice. In the Rag2pGFP model, immature (CD24+) ?? thymocytes expressed high levels of GFP whereas only a minority of mature (CD24-) ?? thymocytes were GFP+ Similarly, most peripheral GFP+ ?? T cells were immature. Analysis of ?? recent thymic emigrants (RTEs) indicated that most ?? T cell RTEs were CD24+ and GFP+, and adoptive transfer experiments demonstrated that immature ?? thymocytes can mature outside the thymus. Mature ?? T cells largely did not recirculate to the thymus from the periphery; rather, a population of mature ?? thymocytes that produced IFN-? or IL-17 remained resident in the thymus for at least 60 d. These data support the existence of two populations of ?? T cell RTEs in adult mice: a majority subset that is immature and matures in the periphery after thymic emigration, and a minority subset that completes maturation within the thymus prior to emigration. Additionally, we identified a heterogeneous population of resident ?? thymocytes of unknown functional importance. Collectively, these data shed light on the generation of the ?? T cell compartment in adult mice.
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更多信息

更多信息
Species Non-Human Primate, Other, Rat
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Bone Marrow, Buffy Coat, Cord Blood, Leukapheresis, Other, PBMC, Peripheral Blood, Spleen
Selection Method Positive
Legal Statement: Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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ISO 14001 环境管理体系认证

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