总览

使用 EasySep™ Direct 人CTC富集试剂盒，可通过免疫磁珠负选，简便高效地从人全血样本中直接分选出高纯度的人循环肿瘤细胞（CTCs）。EasySep™ 技术结合了单克隆抗体的特异性和无柱磁选系统的简便性，在过去 20 多年中被广泛应用于发表的科学研究中。

在此 EasySep™ 负选过程中，非目的细胞会被抗体复合物和EasySep™ Direct

RapidSpheres™ 的磁珠标记。表达以下标志物的非目标细胞将被去除：CD2、CD14、CD16、CD19、CD45、CD61 和 CD66b。通过EasySep™磁极将被磁性标记的细胞与未被标记的目的细胞CTCs中分离出来，只需简单地将目的细胞倾倒或吸取至新的分离管中即可。完成磁珠细胞分选后，目的CTCs即可用于流式细胞术、细胞培养或DNA/RNA提取等下游应用。了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多可优化您的实验流程的相关产品，包括细胞鉴定、冻存及其他产品。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)

亚型
细胞分选试剂盒

细胞类型
癌细胞及细胞系

种属
人

样本来源
Whole Blood

筛选方法
Negative

应用
细胞分选

品牌
EasySep

研究领域
癌症，免疫

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Data Figures

$CAMA cells were seeded into whole blood at a starting frequency of 0.98%. The CAMA cell (EpCAM+) content of the enriched fraction is 92.02% with a 3.8 log depletion of CD45+ cells$

Figure 1. Typical EasySep™ Direct Human CTC Enrichment Profile

Starting with human whole blood from healthy donors, spiked with approximately 1% of CAMA cells (epithelial tumor cell line), the typical CTC (epithelial cell+) content of non-lysed final enriched fraction is 79 ± 16 % (using the silver “Big Easy” EasySep™ Magnet; gated on DRAQ5™ for nucleated cells). Typically the log depletion of targeted CD45+ cells is 2.8 to 3.2. In the above example, CAMA cells were seeded into whole blood at a starting frequency of 0.98%. The CAMA cell (epithelial cell+) content of the enriched fraction is 92.02% with a 3.8 log depletion of CD45+ cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

EasySep™ Direct Human CTC Enrichment Kit

Catalog #

19657

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Direct Human CTC Enrichment Kit

Catalog #

19657

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Direct Human CTC Enrichment Kit

Catalog #

19657

Lot #

All

Language

English

Resources and Publications

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

Facile, rapid and efficient isolation of circulating tumor cells using aptamer-targeted magnetic nanoparticles integrated with a microfluidic device. A. A. Kajani et al. RSC advances 2022 nov

Abstract

Facile and sensitive detection and isolation of circulating tumor cells (CTCs) was achieved using the aptamer-targeted magnetic nanoparticles (Apt-MNPs) in conjugation with a microfluidic device. Apt-MNPs were developed by the covalent attachment of anti-MUC1 aptamer to the silica-coated magnetic nanoparticles via the glutaraldehyde linkers. Apt-MNPs displayed high stability and functionality after 6 months of storage at 4 °C. The specific microfluidic device consisting of mixing, sorting and separation modules was fabricated through conventional photo- and soft-lithography by using polydimethylsiloxane. The capture efficiency of Apt-MNPs was first studied in vitro on MCF-7 and MDA-MB-231 cancer cell lines in the bulk and microfluidic platforms. The cell capture yields of more than 91% were obtained at the optimum condition after 60 minutes of exposure to 50 $\mu$g mL-1 Apt-MNPs with 10 to 106 cancer cells in different media. CTCs were also isolated efficiently from the blood samples of breast cancer patients and successfully propagated in vitro. The isolated CTCs were further characterized using immunofluorescence staining. The overall results indicated the high potential of the present method for the detection and capture of CTCs.

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Label-free detection of rare circulating tumor cells by image analysis and machine learning. S. Wang et al. Scientific reports 2020

Abstract

Detection and characterization of rare circulating tumor cells (CTCs) in patients' blood is important for the diagnosis and monitoring of cancer. The traditional way of counting CTCs via fluorescent images requires a series of tedious experimental procedures and often impacts the viability of cells. Here we present a method for label-free detection of CTCs from patient blood samples, by taking advantage of data analysis of bright field microscopy images. The approach uses the convolutional neural network, a powerful image classification and machine learning algorithm to perform label-free classification of cells detected in microscopic images of patient blood samples containing white blood cells and CTCs. It requires minimal data pre-processing and has an easy experimental setup. Through our experiments, we show that our method can achieve high accuracy on the identification of rare CTCs without the need for advanced devices or expert users, thus providing a faster and simpler way for counting and identifying CTCs. With more data becoming available in the future, the machine learning model can be further improved and can serve as an accurate and easy-to-use tool for CTC analysis.

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EasySep™ Direct人CTC富集试剂盒

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产品号 #19657_C

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Scientists Helping Scientists™

Species	Human
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
Sample Source	Whole Blood
Selection Method	Negative

EasySep™ Direct人CTC富集试剂盒

产品号 #（选择产品）

产品号 #19657_C

产品优势

产品组分包括

总览

Data Figures

Protocols and Documentation

Resources and Publications

Educational Materials (11)

Frequently Asked Questions

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Publications (2)

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Abstract

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