产品号 #60132_C
小鼠单克隆IgG1抗体，抗恒河猴红细胞
总览
小鼠单克隆抗体T3G6识别恒河猴骨髓中的成熟红细胞和红系前体细胞。抗体克隆T3G6基于用恒河猴（Macaca mulatta）红细胞免疫的小鼠开发。克隆T3G6所结合的抗原尚未确定，与其他种类猴子红细胞的反应性也尚未确定。克隆T3G6不能识别人红细胞。
亚型
一抗
靶抗原
Red Blood Cell
别名
红细胞，红细胞
活性物种
非人灵长类
偶联
未偶联的
宿主物种
小鼠
细胞类型
红系细胞，红细胞
种属
非人灵长类
应用
细胞分选，流式细胞术
研究领域
免疫
克隆
T3G6
基因编号
Not available
同种型
IgG1，kappa
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Development of a human immunodeficiency virus type 1-based lentiviral vector that allows efficient transduction of both human and rhesus blood cells. Journal of virology 2009 OCT
Abstract
Human immunodeficiency virus type 1 (HIV-1) vectors transduce rhesus blood cells poorly due to a species-specific block by TRIM5alpha and APOBEC3G, which target HIV-1 capsid and viral infectivity factor (Vif), respectively. We sought to develop a lentiviral vector capable of transducing both human and rhesus blood cells by combining components of both HIV-1 and simian immunodeficiency virus (SIV), including SIV capsid (sCA) and SIV Vif. A chimeric HIV-1 vector including sCA (chiHIV) was superior to the conventional SIV in transducing a human blood cell line and superior to the conventional HIV-1 vector in transducing a rhesus blood cell line. Among human CD34(+) hematopoietic stem cells (HSCs), the chiHIV and HIV-1 vectors showed similar transduction efficiencies; in rhesus CD34(+) HSCs, the chiHIV vector yielded superior transduction rates. In in vivo competitive repopulation experiments with two rhesus macaques, the chiHIV vector demonstrated superior marking levels over the conventional HIV-1 vector in all blood lineages (first rhesus, 15 to 30% versus 1 to 5%; second rhesus, 7 to 15% versus 0.5 to 2%, respectively) 3 to 7 months postinfusion. In summary, we have developed an HIV-1-based lentiviral vector system that should allow comprehensive preclinical testing of HIV-1-based therapeutic vectors in the rhesus macaque model with eventual clinical application.
更多信息
| Species | Non-Human Primate |
|---|---|
| Clone | T3G6 |
| Gene Id | Not available |
| Alternative Names | Erythrocyte, RBC |
| Isotype | IgG1, kappa |
相关产品
-
小鼠 IgG1，kappa 同型对照抗体（克隆 MOPC-21）小鼠单克隆IgG1， kappa同型对照抗体
-
小鼠IgG1, kappa同型对照抗体，克隆X40小鼠单克隆IgG1， kappa同型对照抗体
-
抗恒河猴红细胞抗体，克隆T4G6小鼠单克隆IgG2a抗体，抗恒河猴红细胞
-
山羊抗小鼠 IgG (H+L) 抗体，多克隆抗小鼠IgG（H+L）山羊多克隆抗体
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
