产品号 #60051_C
抗小鼠、大鼠巢蛋白的小鼠单克隆IgG1抗体
Rat401抗体与小鼠和大鼠Nestin,这是一种分子量大于170 kDa的VI类中间纤维(IF)蛋白,在神经上皮干细胞和祖细胞以及一些其他细胞类型中表达,包括胰岛祖细胞、血管内皮细胞、神经胶质瘤细胞和骨髓间充质干细胞。在神经分化过程中,Nestin 表达逐渐下调;在成人神经系统中,其表达主要局限于皮层脑室下区、海马齿状回、背根神经节的卫星细胞以及施旺细胞的一个亚群中。Nestin 通过依赖磷酸化的机制与波形蛋白(vimentin)或 α-internexin 形成异二聚体,从而组装成中间丝结构,参与细胞的结构组织。它对神经祖细胞存活、更新和增殖至关重要,也可能参与神经元分化过程中生长锥的引导。在分化过程中,含 nestin 的中间丝会被诸如 GFAP 等细胞类型特异性的中间丝所取代。
该抗体克隆已通过验证,可用于标记使用 NeuroCult™ 增殖试剂盒(小鼠和大鼠;产品号:# 05702)培养的神经干细胞和祖细胞。
亚型
一抗
靶抗原
Nestin
别名
NES,VI型中间丝(IF)蛋白
活性物种
小鼠,大鼠
偶联
未偶联的
宿主物种
小鼠
细胞类型
神经元
种属
小鼠,大鼠
应用
免疫细胞化学,免疫荧光,免疫组化,Western印迹
研究领域
神经科学
克隆
Rat401
基因编号
18008
同种型
IgG1,kappa
Figure 1. Data for Unconjugated
(A) Embryonic mouse cortical tissue was cultured using the NeuroCult™ Proliferation Kit (Mouse), then fixed and labeled with Anti-Rat Nestin Antibody, Clone Rat401, followed by goat anti-mouse IgG, FITC. Nuclei were counter-stained with DAPI. Inset shows cells labeled with a mouse IgG1, kappa isotype control antibody (Anti-Dextran Antibody, Clone DX1, Catalog #60026) followed by goat anti-mouse IgG, FITC (without DAPI staining).
(B) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit, then fixed and labeled with Anti-Rat Nestin Antibody, Clone Rat401, followed by goat anti-mouse IgG, FITC. Nestin expression is down-regulated during neuronal differentiation. Inset shows cells labeled with a positive control antibody (anti-neuronal class III beta-tubulin antibody) followed by goat anti-mouse IgG, FITC.
(C) Western blot analysis of denatured/reduced cell lysates with Anti-Rat Nestin Antibody, Clone Rat401. Lane 1, mouse neural progenitor cells cultured with the NeuroCult™ Proliferation Kit (Mouse), lane 2, HT1080 fibrosarcoma cells (negative control), lane 3, adult rat brain cells (negative control).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Species | Mouse, Rat |
---|---|
Clone | Rat401 |
Gene Id | 18008 |
Alternative Names | NES, Type VI intermediate filament (IF) protein |
Isotype | IgG1, kappa |
小鼠和大鼠神经干细胞及祖细胞扩增培养基套装
小鼠单克隆IgG1, kappa同型对照抗体
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