产品号 #60060_C
抗人、小鼠、大鼠SSEA-1（CD15）的小鼠单克隆IgM抗体
总览
MC-480抗体与末端碳水化合物表位，阶段特异性胚胎抗原-1 （SSEA-1）反应，SSEA-1表达于小鼠早期胚胎，小鼠胚胎癌（EC），胚胎干细胞（ES）和小鼠及人胚胎生殖（EG）细胞表面的一种大分子量（> 200 kDa）糖蛋白上。SSEA-1在未分化的人EC、ES或诱导多能干细胞（iPS）或恒河猴ES细胞系上不表达。其在小鼠胚胎干细胞中的表达在分化过程中降低，而在人ES细胞中，其表达在分化过程中会上调。SSEA-1也存在于成人粒细胞和单核细胞中，标记为CD15， MC-480抗体识别这些细胞类型上的CD15标记。据报道，SSEA-1在细胞粘附和迁移以及细胞分化调控中发挥作用。
该抗体克隆已通过EasySep™试剂盒分离的细胞纯度评估验证，包括EasySep™HLA全血CD15正选试剂盒(产品号#18681HLA；可观察到部分阻断)，并用于标记在TeSR™-E8™（产品号#05940），mTeSR™1（产品号#85850）和TeSR™2（产品号#05860）中生长的人 ES和iPS细胞。
亚型
一抗
靶抗原
SSEA-1 (CD15)
别名
3-FAL，CD15，Lewis X，SSEA1，阶段特异性胚胎抗原1，X-半抗原
活性物种
人，小鼠，大鼠
偶联
Alexa Fluor 488，Biotin 或 生物素，FITC，PE，未偶联的
宿主物种
小鼠
细胞类型
多能干细胞
种属
人，小鼠，大鼠
应用
细胞分选，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
研究领域
干细胞生物学
克隆
MC-480
基因编号
14345
同种型
IgM，kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (3)
Publications (1)
OCT4 as a target of miR-34a stimulates p63 but inhibits p53 to promote human cell transformation Cell death & disease 2014 JAN
Abstract
Human cell transformation is a key step for oncogenic development, which involves multiple pathways; however, the mechanism remains unclear. To test our hypothesis whether cell oncogenic transformation shares some mechanisms with the process of reprogramming non-stem cells to induced pluripotent stem cells (iPSC), we studied the relationship among the key factors for promoting or inhibiting iPSC in radiation-transformed human epithelial cell lines derived from different tissues (lung, breast and colon). We unexpectedly found that p63 and OCT4 were highly expressed (accompanied by low expressed p53 and miR-34a) in all transformed cell lines examined when compared with their non-transformed counterparts. We further elucidated the relationship of these factors: the 3p strand of miR-34a directly targeted OCT4 by binding to the 3′ untranslated region (3′-UTR) of OCT4 and, OCT4, in turn, stimulated p63 but inhibited p53 expression by binding to a specific region of the p63 or p53 promoter. Moreover, we revealed that the effects of OCT4 on promoting cell oncogenic transformation were by affecting p63 and p53. These results support that a positive loop exists in human cells: OCT4 upregulation as a consequence of inhibition of miR-34a, promotes p63 but suppresses p53 expression, which further stimulates OCT4 upregulation by downregulating miR-34a. This functional loop contributes significantly to cell transformation and, most likely, also to the iPSC process.
更多信息
| Species | Human, Mouse, Rat |
|---|---|
| Clone | MC-480 |
| Gene Id | 14345 |
| Alternative Names | 3-FAL, CD15, Lewis X, SSEA1, Stage-specific embryonic antigen 1, X-hapten |
| Isotype | IgM, kappa |
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