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抗小鼠Sca1抗体，克隆E13-161.7

大鼠抗小鼠Sca1 (Ly-6A/E)单克隆IgG2a抗体
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产品号 #60032_C

大鼠抗小鼠Sca1 (Ly-6A/E)单克隆IgG2a抗体

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实验数据
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应用领域
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总览

E13-161.7 单克隆抗体可识别 Sca1（干细胞抗原-1，或称 Ly-6A/E），这是一种分子量约为 18 kDa 的糖基磷脂酰肌醇（GPI）锚定蛋白，属于淋巴细胞活化蛋白-6（Ly-6）家族。Sca1表达于造血干细胞与祖细胞、髓系细胞以及外周 B 和 T 淋巴细胞的细胞表面。

在携带Ly-6.1或Ly-6.2等位基因的小鼠中均可检测到Sca1的表达，但依照等位基因型的不同，在外周血循环细胞中Sca1的表达模式有所不同。携带 Ly-6.2 等位基因的小鼠品系（如 AKR、C57BL、C57BR、C57L、DBA/2、PL、SJL、SWR、129）相比于携带 Ly-6.1 的品系（如 A、BALB/c、CBA、C3H/He、DBA/1、NZB）拥有相对较多数量的 Sca1+ 静息淋巴细胞。

在所有小鼠品系中，细胞激活后 Sca1 的表达水平都会显著上调。Sca1 参与调控 T 细胞与 B 细胞的免疫应答，并被认为在多种干细胞的分化、增殖与存活过程中发挥重要作用。Sca1 已成为鉴定和分选造血干细胞及祖细胞的优选表型标志物。

该抗体克隆已被验证可用于评估使用 EasySep™试剂盒（包括 EasySep™小鼠Sca1生物素阳选试剂盒，产品号 #18856）分选获得的细胞的纯度。

亚型
一抗
 
靶抗原
Sca1 (Ly-6A/E)
 
别名
Ly-6A/E，Sca-1
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
造血干/祖细胞，乳腺细胞
 
种属
小鼠
 
应用
细胞分选，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
上皮细胞研究，干细胞生物学
 
克隆
E13-161.7
 
基因编号
110454
 
同种型
IgG2a，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells pre-labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488 and processed with the EasySep™ Mouse SCA1 Positive Selection Kit. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ PE Selection Kit for Mouse Cells (Catalog #18554) using Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2a, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC (filled histogram) or a rat IgG2a, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a biotinylated rat IgG2a, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC (filled histogram) or a rat IgG2a, kappa isotype control antibody, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488
Catalog #
60032AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC
Catalog #
60032AZ, 60032AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin
Catalog #
60032BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC
Catalog #
60032FI.1, 60032FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE
Catalog #
60032PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7
Catalog #
60032
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488
Catalog #
60032AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC
Catalog #
60032AZ, 60032AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin
Catalog #
60032BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC
Catalog #
60032FI.1, 60032FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE
Catalog #
60032PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7
Catalog #
60032
Lot #
All
Language
English

更多信息

更多信息
Species Mouse
Clone E13-161.7
Gene Id 110454
Alternative Names Ly-6A/E, Sca-1
Isotype IgG2a, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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