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抗小鼠CD117抗体，克隆2B8

大鼠单克隆IgG2b抗体，抗小鼠CD117（c-Kit）
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产品号 #60025_C

大鼠单克隆IgG2b抗体，抗小鼠CD117（c-Kit）

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

2B8抗体识别CD117 （c-Kit），CD117 （c-Kit）是一种~145 kDa的c-Kit配体（干细胞因子/钢铁因子）的1型跨膜受体，广泛表达于骨髓造血干细胞，包括多能祖细胞、红系祖细胞、B和T淋巴细胞前体，以及肥大细胞。CD117属于酪氨酸激酶受体家族，具有5个免疫球蛋白样C2型结构域和1个细胞质蛋白激酶结构域。c-Kit配体与CD117结合可诱导二聚化和自磷酸化，从而激活对造血干细胞增殖和分化至关重要的几种细胞内信号通路。CD117信号通过磷酸酶和受体的快速内吞和降解来调控。2B8抗体不会阻断c-Kit配体的结合。CD117的突变与各种类型的肿瘤和骓斑（一种常染色体显性色素沉着异常）有关。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠造血祖细胞分选试剂盒（产品号 #19856））分选的细胞纯度。

亚型
一抗
 
靶抗原
CD117 (c-Kit)
 
别名
c-KIT，cKIT，干细胞因子受体（SCFR）
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
造血干/祖细胞
 
种属
小鼠
 
应用
细胞分选，流式细胞术，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
干细胞生物学
 
克隆
2B8
 
基因编号
16590
 
同种型
IgG2b，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488 and anti-mouse CD45R APC. (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells pre-labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488 and processed with the EasySep™ Mouse CD117 Positive Selection Kit (Catalog #18757). Histograms show labeling of bone marrow (Start) and isolated cells (Isolated); partial blocking may be observed. Labeling with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, PE and anti-mouse CD45R APC. (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse PE Positive Selection Kit (Catalog #18554), and labeled with Anti-Mouse CD117 Antibody, Clone 2B8, PE. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling with a rat IgG2b, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 (c-Kit) Antibody, Clone 2B8, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin (Catalog #60077BT), followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone 2B8, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488
Catalog #
60025AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, APC
Catalog #
60025AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, Biotin
Catalog #
60025BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, PE
Catalog #
60025PE, 60025PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8
Catalog #
60025
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, Alexa Fluor® 488
Catalog #
60025AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, APC
Catalog #
60025AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, Biotin
Catalog #
60025BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8, PE
Catalog #
60025PE, 60025PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone 2B8
Catalog #
60025
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse
Clone 2B8
Gene Id 16590
Alternative Names c-KIT, cKIT, Stem cell factor receptor (SCFR)
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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