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抗小鼠MHC II类（I-A/I-E）抗体，克隆M5/114.15.2，APC

抗小鼠MHC II类（I-A/I-E）的大鼠单克隆IgG2b抗体
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产品号 #100-0290_C

抗小鼠MHC II类（I-A/I-E）的大鼠单克隆IgG2b抗体

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总览
实验数据
产品说明书及文档
应用领域
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总览

M5/114.15.2（M5/114）抗体可特异性识别小鼠主要组织相容性复合物II类受体（MHC-II）上的胞外表位。MHC-II是一种异二聚跨膜糖蛋白，由一个α亚基与一个β亚基非共价结合而成。在小鼠中，MHC-II复合物被命名为I-A和I-E，分别包含Aα/Aβ和Eα/Eβ异源二聚体。MHC-II与CD3/T细胞受体（TCR）复合物以及CD4分子一起，介导抗原递呈细胞将外源抗原水解产生的肽递呈给CD4+ T（辅助性）细胞的关键功能，从而抑制或诱导对肽的免疫反应。M5/114.15.2抗体可识别I-Ab、I-Ad、I-Aq、I-Ed和I-Ek（但不包括I-Af、I-Ak或I-As） MHC II类同种抗原所共有的多态性决定因素，这些抗原在携带H-2b、d、p、q、r和u单倍型（但不包括H-2f或H-2s单倍型）的小鼠B细胞、树突状细胞、巨噬细胞、单核细胞和激活的T细胞上表达。研究发现，M5/114抗体抑制H-2b、d、q和u单倍型T细胞的I-A限制性反应（但不抑制H-2f、k或s单倍型），与NOD （H-2g7）小鼠细胞不发生反应。

亚型
一抗
 
靶抗原
MHC Class II (I-A/I-E)
 
别名
IA，I-A，IA/IE，I-A/E，I-E，MHC class II，MHC-II
 
活性物种
小鼠
 
偶联
APC，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
B 细胞，巨噬细胞，单核细胞，T 细胞
 
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，放射免疫分析，Western印迹
 
研究领域
免疫
 
克隆
M5/114.15.2
 
基因编号
14961/14969
 
同种型
IgG2b，kappa
 

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Data Figures

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC or a rat IgG2b, kappa APC isotype control antibody.

Figure 1. Data for Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC (filled histogram) or a rat IgG2b, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC or a rat IgG2b, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, FITC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC (filled histogram) or a rat IgG2b, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE or a rat IgG2b, kappa PE isotype control antibody.

Figure 3. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, PE

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE.

Figure 4. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2,Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE (filled histogram) or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, APC
Catalog #
100-0291
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, FITC
Catalog #
100-0295
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, PE
Catalog #
100-0292
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2
Catalog #
100-0297
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, APC
Catalog #
100-0291
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, FITC
Catalog #
100-0295
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2, PE
Catalog #
100-0292
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2
Catalog #
100-0297
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Clone M5/114.15.2
Gene Id 14961/14969
Alternative Names IA, I-A, IA/IE, I-A/E, I-E, MHC class II, MHC-II
Isotype IgG2b, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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