产品号 #60028_C
Rat monoclonal IgG2b antibody against mouse Gr-1 (Ly-6G/Ly-6C)
概述
The RB6-8C5 antibody reacts with the structurally related murine Ly-6G (21 - 25 kDa) and Ly-6C (14 - 16 kDa) GPI-anchored proteins, which together comprise the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils, subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. Whereas the RB6-8C5 binds to both Ly-6G and Ly-6C, another commonly used anti-Gr-1 antibody, clone 1A8, binds specifically to Ly-6G. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes and dendritic cells.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).
Subtype
Primary Antibodies
Target Antigen
Gr-1 (Ly-6G/Ly-6C)
Alternative Names
Gr 1, Gr1, Ly 6G, Ly6G, Ly-6G/Ly-6C
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Granulocytes and Subsets, Monocytes, Myeloid Cells
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
RB6-8C5
Gene ID
17067/546644
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
Figure 2. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2b, kappa isotype control antibody, followed by SAV APC (solid line histogram).
Figure 3. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).
Figure 4. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a rat IgG2b, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC is shown (solid line histogram).
Figure 5. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).
Figure 6. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).
Figure 7. Data for PerCP-Cy5.5-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on granulocytes) labeled with Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC Catalog #
60028AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin Catalog #
60028BT, 60028BT.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC Catalog #
60028FI.1, 60028FI Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE Catalog #
60028PE, 60028PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5 Catalog #
60028PS Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5 Catalog #
60028 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, APC Catalog #
60028AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, Biotin Catalog #
60028BT, 60028BT.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, FITC Catalog #
60028FI.1, 60028FI Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PE Catalog #
60028PE, 60028PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5, PerCP-Cy5.5 Catalog #
60028PS Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Gr-1 Antibody, Clone RB6-8C5 Catalog #
60028 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Granulocyte Research
Resources and Publications
Educational Materials (4)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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