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抗小鼠EPCR抗体，克隆RMEPCR1560 （1560）

大鼠抗小鼠EPCR （CD201）单克隆IgG2b抗体
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产品号 #60038_C

大鼠抗小鼠EPCR （CD201）单克隆IgG2b抗体

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概述

RMEPCR1560（1560）抗体与内皮蛋白C受体（EPCR或CD201）反应，内皮细胞、造血干细胞亚群和树突状细胞以及几种恶性细胞系表达约25 kDa的I型跨膜糖蛋白。它也以可溶性形式存在于血浆中。EPCR与MHC 1/CD1蛋白家族具有同源性。EPCR结合蛋白C和活化蛋白C，通过凝血酶-血栓调节蛋白复合物增强蛋白C的活化，调节血液凝固和炎症。约1.5%的小鼠骨髓细胞中检测到EPCR蛋白的表达。纯化的EPCR+细胞具有高度富集的HSC活性，证明了高的体内再生活性。此外，EPCR的表达与使用常规标记分离的骨髓细胞群的干细胞活性相关，这表明EPCR作为鉴定小鼠造血干细胞的单一标记物是有用的。

Subtype
Primary Antibodies
 
Target Antigen
EPCR (CD201)
 
Alternative Names
Activated protein C receptor, APC receptor, CCD41, CD201, endothelial protein C receptor, PROCR, protein C receptor
 
Reactive Species
Mouse
 
Conjugation
Biotin, FITC, PE, Unconjugated
 
Host Species
Rat
 
Cell Type
Endothelial Cells, Hematopoietic Stem and Progenitor Cells
 
Species
Mouse
 
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blotting
 
Area of Interest
Endothelial Cell Biology, Stem Cell Biology
 
Clone
RMEPCR1560 (1560)
 
Gene ID
19124
 
Isotype
IgG2b, kappa
 

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Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of HEK-293 mEPCR-transfected cells (filled histogram) or non-transfected HEK-293 cells (negative control; dashed line histogram), labeled with Anti-Mouse EPCR Antibody, Clone RMEPCR1560, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI). Labeling of HEK-293 mEPCR-transfected cells with a rat IgG2b, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 2. Data for Biotin-Conjugated

Flow cytometry analysis of HEK-293 mEPCR-transfected cells (filled histogram) or non-transfected HEK-293 cells (negative control cells, dashed line histogram), labeled with Anti-Mouse EPCR Antibody, Clone RMEPCR1560, Biotin followed by streptavidin (SAV) APC. Labeling of HEK-293 mEPCR-transfected cells with a rat IgG2b, kappa isotype control antibody followed by SAV APC is shown (solid line histogram).

Data for FITC-Conjugated

Figure 3. Data for FITC-Conjugated

Flow cytometry analysis of HEK-293 mEPCR-transfected cells (filled histogram) or non-transfected HEK-293 cells (negative control cells, dashed line histogram), labeled with Anti-Mouse EPCR Antibody, Clone RMEPCR1560 FITC. Labeling of HEK-293 mEPCR-transfected cells with a rat IgG2b, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of HEK-293 mEPCR-transfected cells (filled histogram) or non-transfected HEK-293 cells (negative control cells, dashed line histogram), labeled with Anti-Mouse EPCR Antibody, Clone RMEPCR1560, PE. Labeling of HEK-293 mEPCR-transfected cells with a rat IgG2b, kappa PE isotype control antibody is shown (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), FITC
Catalog #
100-1576
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), FITC
Catalog #
60038FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560)
Catalog #
60038
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), PE
Catalog #
60038PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), Biotin
Catalog #
60038BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), FITC
Catalog #
100-1576, 60038FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560)
Catalog #
60038
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), PE
Catalog #
60038PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse EPCR Antibody, Clone RMEPCR1560 (1560), Biotin
Catalog #
60038BT
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse
Clone RMEPCR1560 (1560)
Gene Id 19124
Alternative Names Activated protein C receptor, APC receptor, CCD41, CD201, endothelial protein C receptor, PROCR, protein C receptor
Isotype IgG2b, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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