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抗小鼠CD150抗体，克隆TC15-12F12.2

抗小鼠CD150（SLAM）大鼠单克隆IgG2a抗体
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产品号 #60036_C

抗小鼠CD150（SLAM）大鼠单克隆IgG2a抗体

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实验数据
产品说明书及文档
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总览

TC15-12F12.2抗体与CD150（信号淋巴细胞活化分子，SLAM）发生反应。CD150是一种分子量约为75 kDa的I型跨膜糖蛋白，作为细胞粘附分子和/或辅助受体，在免疫反应中发挥多种作用。它在T细胞、未成熟胸腺细胞、B细胞、树突状细胞、巨噬细胞和内皮细胞中存在差异表达。其表达模式因细胞类型和活化状态而异。T细胞、B细胞和树突状细胞活化后，CD150会快速诱导表达，且T细胞的合成主要在Th1细胞克隆上进行，而非Th2细胞克隆。CD150介导的TCR激活T细胞的共刺激作用可增强Th1细胞产生IFN-γ，而这种应答可通过TC15-12F12.2抗体的结合得到增强。 CD150被认为通过与细胞内蛋白酪氨酸磷酸酶SHP-2结合来介导信号转导。CD150还在造血细胞发育中发挥作用，并且是检测多能造血干细胞的有效标记物（与CD48和CD41等其他标记物协同作用）。CD150在非多能造血祖细胞上不表达。

亚型
一抗
 
靶抗原
CD150 (SLAM)
 
别名
IPO-3，信号淋巴细胞活化分子
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，PE，PE-Cyanine7，未偶联的
 
宿主物种
Hamster，大鼠
 
细胞类型
造血干/祖细胞
 
种属
小鼠
 
应用
流式细胞术，功能学筛选，免疫荧光，免疫组化，免疫沉淀
 
研究领域
干细胞生物学
 
克隆
TC15-12F12.2
 
基因编号
27218
 
同种型
IgG2a，lambda
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, lambda Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE (filled histogram) or a rat IgG2a, lambda PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE (filled histogram) or a rat IgG2a, lambda PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or a rat IgG2a isotype control antibody followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, APC (filled histogram) or a rat IgG2a isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2a isotype control antibody followed by SAV APC (solid line histogram).

(A) Primary human airway epithelial cells were cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface, then cryo-sectioned and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by a goat anti-rabbit IgG antibody, Alexa Fluor® 594 (red), and an anti-human NGF Receptor/p75NTR (CD271) antibody, followed by a donkey anti-mouse IgG antibody, Alexa Fluor® 488 (green). (B) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, followed by an anti-mouse IgG1 antibody, PE and anti-human CD3 antibody, Clone HIT3a, Pacific Blue™. (C) Flow cytometry analysis of human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by an anti-mouse IgG1 antibody, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

(A) Flow cytometry analysis of human airway epithelial cells cultured in PneumaCult™-ALI Medium (Catalog #05001) at the air-liquid interface. Cells were enzymatically dissociated and labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE, solid line histogram). (C) Flow cytometry analysis human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Anti-Human MUC1 (CD227) Antibody, Clone 16A, PE and anti-human CD3 antibody, clone HIT3a, Pacific Blue™. (D) Flow cytometry analysis of PHA-activated human peripheral blood lymphocytes following stimulation with PHA for 3 days. Cells were labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE, and anti-human CD3 antibody, clone HIT3a, Pacific Blue™.

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE-Cyanine7 (filled histogram) or a rat IgG2a isotype control antibody, PE-Cyanine7 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Alexa Fluor® 488
Catalog #
60036AD, 60036AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, APC
Catalog #
60036AZ, 60036AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Biotin
Catalog #
60036BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE
Catalog #
60036PE, 60036PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE-Cyanine7
Catalog #
60036CZ, 60036CZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2
Catalog #
60036.1, 60036
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Alexa Fluor® 488
Catalog #
60036AD, 60036AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, APC
Catalog #
60036AZ, 60036AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, Biotin
Catalog #
60036BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE
Catalog #
60036PE, 60036PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2, PE-Cyanine7
Catalog #
60036CZ, 60036CZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD150 Antibody, Clone TC15-12F12.2
Catalog #
60036.1, 60036
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse
Clone TC15-12F12.2
Gene Id 27218
Alternative Names IPO-3, Signaling lymphocyte activation molecule
Isotype IgG2a, lambda
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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