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抗鼠CD8a抗体，克隆53-6.7

抗小鼠、蟾蜍CD8a的大鼠单克隆IgG2a抗体
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产品号 #60023_C

抗小鼠、蟾蜍CD8a的大鼠单克隆IgG2a抗体

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

53-6.7抗体可特异性识别小鼠CD8a，CD8a是一种32 - 34 kDa的I型跨膜糖蛋白，是CD8的一个亚基。CD8是一种二硫键结合的二聚体，可以是CD8a （α）和CD8b （β）亚基的异二聚体（即αβ）形式或同二聚体（αα）形式。CD8作为T细胞受体（TCR）的共受体，在T细胞活化过程中结合抗原递呈细胞递呈的MHC I类分子。它的作用是加强TCR和MHC I-抗原复合物之间的结合，并通过胞内结构域与胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到细胞质的信号。CD8a链与MHC I类分子的α -3结构域结合。CD8在大多数胸腺细胞和一部分成熟的外周血T细胞（毒性T细胞）中以αβ形式表达，在γδ T细胞（肠上皮内淋巴细胞和树突状细胞的亚群）中以ββ形式表达。研究发现，53-6.7抗体可阻断通过MHC I类抗原的递呈，并抑制IL-2依赖性T细胞应答。该抗体克隆已验证可用于EasySep™试剂盒分离的细胞的纯度评估，包括EasySep™小鼠CD8+ T细胞分选试剂盒（产品号 #19853）和EasySep™小鼠CD90.2正选试剂盒II（产品号 #18951）。

亚型
一抗
 
靶抗原
CD8a
 
别名
Ly-2，Lyt2，T8
 
活性物种
小鼠，Toad
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
T 细胞，T 细胞，CD8+
 
种属
小鼠，其它细胞系
 
应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
免疫
 
克隆
53-6.7
 
基因编号
12525
 
同种型
IgG2a，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (Catalog #60015PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) and Anti-Mouse CD3e Antibody, Clone 145-2C11, PE.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488
Catalog #
60023AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, APC
Catalog #
60023AZ, 60023AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin
Catalog #
60023BT.1, 60023BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC
Catalog #
60023FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, PE
Catalog #
60023PE, 60023PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5
Catalog #
60023PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, Alexa Fluor® 488
Catalog #
60023AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, APC
Catalog #
60023AZ, 60023AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, Biotin
Catalog #
60023BT.1, 60023BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, FITC
Catalog #
60023FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, PE
Catalog #
60023PE, 60023PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD8a Antibody, Clone 53-6.7, PerCP-Cy5.5
Catalog #
60023PS.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse, Other
Clone 53-6.7
Gene Id 12525
Alternative Names Ly-2, Lyt2, T8
Isotype IgG2a, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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