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抗小鼠CD4抗体，克隆RM4-5

大鼠单克隆IgG2a抗体，抗小鼠CD4
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产品号 #（选择产品）

产品号 #60017_C

大鼠单克隆IgG2a抗体，抗小鼠CD4

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

RM4-5抗体识别小鼠CD4，CD4是一种~59 kDa的单链1型跨膜糖蛋白，是免疫球蛋白（Ig）超家族的成员。CD4包含4个细胞外Ig样结构域。CD4在大多数胸腺细胞和一些T细胞亚群（辅助/诱导T细胞）中表达水平相对较高，而在树突状细胞中表达水平较低。在小鼠中，单核细胞/巨噬细胞不表达CD4。CD4结合MHC II的非多态性区域，并通过增强TCR与MHC II-抗原复合物之间结合的亲和力，在MHC II限制性抗原识别中作为T细胞受体（TCR）的共受体。CD4还通过胞内结构域与细胞质酪氨酸激酶（如Lck）的相互作用，放大从TCR到胞质的信号。RM4-5抗体与CD4的结合可抑制体外的配体结合；RM4-5抗体的结合可被抗体克隆GK1.5阻断。

该抗体克隆已被验证可用于评估EasySep™试剂盒（包括EasySep™小鼠CD4+ T细胞分选试剂盒（产品号 #19852）和EasySep™小鼠CD25调节性T细胞正选试剂盒（产品号 #18782）分选的细胞纯度。

亚型
一抗
 
靶抗原
CD4
 
别名
L3T4，T4
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
 
宿主物种
大鼠
 
细胞类型
T 细胞，T 细胞，CD4+，T 细胞，调节性细胞
 
种属
小鼠
 
应用
细胞分选，CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
免疫
 
克隆
RM4-5
 
基因编号
12504
 
同种型
IgG2a，kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD; solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE; solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ; solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin followed by streptavidin (SAV) APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT) followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI; solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488
Catalog #
60017AD, 60017AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, APC
Catalog #
60017AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin
Catalog #
60017BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, FITC
Catalog #
60017FI.1, 60017FI
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PE
Catalog #
60017PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5
Catalog #
60017PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5
Catalog #
60017
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Alexa Fluor® 488
Catalog #
60017AD, 60017AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, APC
Catalog #
60017AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, Biotin
Catalog #
60017BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, FITC
Catalog #
60017FI.1, 60017FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PE
Catalog #
60017PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5, PerCP-Cy5.5
Catalog #
60017PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD4 Antibody, Clone RM4-5
Catalog #
60017
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Mouse
Clone RM4-5
Gene Id 12504
Alternative Names L3T4, T4
Isotype IgG2a, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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