产品号 #60037_C
抗人、鼠、恒河猴CD49f（整合素α6）的大鼠单克隆IgG2a抗体
总览
GoH3抗体与CD49f（整合素α6）发生反应，CD49f是一种分子量约为150 kDa的跨膜糖蛋白，可与CD29（整合素β1）或CD104（整合素β4）非共价结合，形成异二聚体受体VLA-6和α6β4，后者可与细胞外基质蛋白层粘连蛋白结合。CD49f是一种二硫键连接的二聚体，包含一条分子量约为120 kDa的重链和一条分子量约为30 kDa的膜结合轻链。该蛋白存在剪接变体，会影响其胞质结构域。CD49f在T细胞、单核细胞、血小板、胎盘滋养层细胞、上皮细胞和内皮细胞表面表达。它参与细胞粘附，并调节参与多种过程的信号通路，包括T细胞的活化和增殖，以及干细胞多能性的分化和维持。 CD49f被认为是筛选小鼠乳腺干细胞和祖细胞的最重要标记。GoH3抗体可与CD49f上的胞外表位发生反应，据报道可在体内阻断整合素α6的功能，并在体外阻断整合素α6与层粘连蛋白的结合。
亚型
一抗
靶抗原
CD49f (Integrin α6)
别名
α6整合素，整合素α6，VLA-6α链
活性物种
Baboon，Capuchin Monkey，Cat，Chimpanzee，Cow，Cynomolgus，Dog，Horse，人，小鼠，Pig，兔，Rhesus，Sheep
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，Pacific Blue，PE，未偶联的
宿主物种
大鼠
细胞类型
乳腺细胞
种属
人，小鼠，非人灵长类，其它细胞系
应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
研究领域
上皮细胞研究，免疫
克隆
GoH3
基因编号
16403/3655
同种型
IgG2a，kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function Stem cells (Dayton, Ohio) 2013
Abstract
Fibroblast growth factor (FGF) signaling plays an important role in embryonic stem cells and adult tissue homeostasis, but the function of FGFs in mammary gland stem cells is less well defined. Both FGFR1 and FGFR2 are expressed in basal and luminal mammary epithelial cells (MECs), suggesting that together they might play a role in mammary gland development and stem cell dynamics. Previous studies have demonstrated that the deletion of FGFR2 resulted only in transient developmental defects in branching morphogenesis. Using a conditional deletion strategy, we investigated the consequences of FGFR1 deletion alone and then the simultaneous deletion of both FGFR1 and FGFR2 in the mammary epithelium. FGFR1 deletion using a keratin 14 promoter-driven Cre-recombinase resulted in an early, yet transient delay in development. However, no reduction in functional outgrowth potential was observed following limiting dilution transplantation analysis. In contrast, a significant reduction in outgrowth potential was observed upon the deletion of both FGFR1 and FGFR2 in MECs using adenovirus-Cre. Additionally, using a fluorescent reporter mouse model to monitor Cre-mediated recombination, we observed a competitive disadvantage following transplantation of both FGFR1/R2-null MECs, most prominently in the basal epithelial cells. This correlated with the complete loss of the mammary stem cell repopulating population in the FGFR1/R2-attenuated epithelium. FGFR1/R2-null MECs were partially rescued in chimeric outgrowths containing wild-type MECs, suggesting the potential importance of paracrine mechanisms involved in the maintenance of the basal epithelial stem cells. These studies document the requirement for functional FGFR signaling in mammary stem cells during development.
更多信息
| Species | Human, Mouse, Non-Human Primate, Other |
|---|---|
| Clone | GoH3 |
| Gene Id | 16403/3655 |
| Alternative Names | α6 integrin, integrin α6, VLA-6α chain |
| Isotype | IgG2a, kappa |
相关产品
-
EpiCult™-B 小鼠培养基用于小鼠乳腺上皮细胞的培养和评估
-
大鼠 IgG2a，kappa 同型对照抗体（克隆 RTK2758）大鼠单克隆IgG2a， kappa同型对照抗体
-
抗小鼠CD24抗体，克隆M1/69大鼠单克隆IgG2b抗体，抗小鼠CD24
Legal Statement: Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
