产品号 #60099_C
大鼠单克隆IgG2b抗体，抗小鼠CD24
总览
M1/69抗体识别小鼠CD24，CD24是一种表达在淋巴细胞、粒细胞、胸腺细胞、红细胞、上皮细胞、神经元和树突状细胞上的~35 - 45 kDa糖蛋白。CD24在T细胞和B细胞发育过程中存在差异表达，在大多数成熟的外周T细胞或浆细胞中均未发现其表达。它在pro-B、pre-B和成熟B细胞上表达，但在B细胞活化后表达大幅下调。CD24参与调节B细胞凋亡，并参与阻止B细胞终末分化为分泌抗体的浆母细胞和浆细胞。CD24作为一种粘附或共刺激分子，通过嗜同相互作用或与P-选择素（CD62P）等配体结合，参与淋巴细胞的活化、增殖和分化。
亚型
一抗
靶抗原
CD24
别名
CD24a，热稳定抗原，HSA，Ly-52，Nectadrin，小细胞肺癌簇4抗原
活性物种
小鼠
偶联
Alexa Fluor 488，APC，Biotin 或 生物素，FITC，PE，PerCP-Cyanine5.5，未偶联的
宿主物种
大鼠
细胞类型
B 细胞，乳腺细胞
种属
小鼠
应用
CyTOF，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，Western印迹
研究领域
上皮细胞研究，免疫
克隆
M1/69
基因编号
12484
同种型
IgG2b，kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Publications (1)
Fibroblast Growth Factor Receptor Signaling Is Essential for Normal Mammary Gland Development and Stem Cell Function Stem cells (Dayton, Ohio) 2013
Abstract
Fibroblast growth factor (FGF) signaling plays an important role in embryonic stem cells and adult tissue homeostasis, but the function of FGFs in mammary gland stem cells is less well defined. Both FGFR1 and FGFR2 are expressed in basal and luminal mammary epithelial cells (MECs), suggesting that together they might play a role in mammary gland development and stem cell dynamics. Previous studies have demonstrated that the deletion of FGFR2 resulted only in transient developmental defects in branching morphogenesis. Using a conditional deletion strategy, we investigated the consequences of FGFR1 deletion alone and then the simultaneous deletion of both FGFR1 and FGFR2 in the mammary epithelium. FGFR1 deletion using a keratin 14 promoter-driven Cre-recombinase resulted in an early, yet transient delay in development. However, no reduction in functional outgrowth potential was observed following limiting dilution transplantation analysis. In contrast, a significant reduction in outgrowth potential was observed upon the deletion of both FGFR1 and FGFR2 in MECs using adenovirus-Cre. Additionally, using a fluorescent reporter mouse model to monitor Cre-mediated recombination, we observed a competitive disadvantage following transplantation of both FGFR1/R2-null MECs, most prominently in the basal epithelial cells. This correlated with the complete loss of the mammary stem cell repopulating population in the FGFR1/R2-attenuated epithelium. FGFR1/R2-null MECs were partially rescued in chimeric outgrowths containing wild-type MECs, suggesting the potential importance of paracrine mechanisms involved in the maintenance of the basal epithelial stem cells. These studies document the requirement for functional FGFR signaling in mammary stem cells during development.
更多信息
| Species | Mouse |
|---|---|
| Clone | M1/69 |
| Gene Id | 12484 |
| Alternative Names | CD24a, Heat stable antigen, HSA, Ly-52, Nectadrin, Small cell lung carcinoma cluster 4 antigen |
| Isotype | IgG2b, kappa |
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