抗小鼠CD44抗体,克隆IM7

抗人、鼠、恒河猴CD44(组织非特异性碱性磷酸酶)的大鼠单克隆IgG2b抗体

产品号 #(选择产品)

产品号 #60068_C

抗人、鼠、恒河猴CD44(组织非特异性碱性磷酸酶)的大鼠单克隆IgG2b抗体

总览

IM7 抗体与 CD44 (Ly-24) 发生反应,CD44 是一种分子量约为 80 - 95 kDa 的 1 型跨膜糖蛋白,参与细胞间和细胞基质相互作用。CD44 在许多细胞表面表达,包括白细胞、肝细胞以及内皮细胞、上皮细胞和间充质细胞。T 细胞和 B 细胞活化后,其表达水平会增加,记忆细胞则表现出 CD44high 表型。CD44 可与多种配体结合,包括透明质酸、胶原蛋白、纤连蛋白、生长因子和金属蛋白酶,从而调节淋巴细胞活化、循环和归巢、白细胞滚动和聚集、造血以及肿瘤转移等过程。许多疾病与 CD44 表达改变或功能障碍有关。已鉴定出多种 CD44 亚型,这些亚型具有选择性剪接、差异性 N- 和 O- 糖基化以及硫酸化,这些特性介导该蛋白质在特定细胞中发挥的功能性作用。IM7 单克隆抗体可与所有 CD44 亚型以及两种鼠类同种型上的胞外表位发生反应。

该抗体克隆已验证可用于 EasySep™ 试剂盒分离细胞的纯度评估,包括 EasySep™ 小鼠 CD4+ T 细胞分离试剂盒(产品号#19852)、EasySep™ 小鼠 CD4+CD62L+ T 细胞分离试剂盒(产品号#18765)和 EasySep™ 人 Naïve  CD4+ T 细胞分离试剂盒(产品号#19555)。

亚型
一抗
 
靶抗原
CD44
 
别名
ECMR III,gp85,H-CAM,Hermes,HUTCH-1,Ly24,Ly-24,Pgp-1
 
活性物种
Baboon,Cat,Chimpanzee,Cow,Cynomolgus,Dog,Horse,人,小鼠,Pig,Rhesus,Squirrel Monkey
 
偶联
Alexa Fluor 488,APC,Biotin 或 生物素,FITC,PE,PerCP-Cyanine5.5,未偶联的
 
宿主物种
大鼠
 
细胞类型
乳腺细胞,T 细胞
 
种属
人,小鼠,非人灵长类,其它细胞系
 
应用
细胞分选,CyTOF,ELISAs,流式细胞术,功能学筛选,免疫细胞化学,免疫荧光,免疫组化,免疫沉淀
 
研究领域
上皮细胞研究,免疫,干细胞生物学
 
克隆
IM7
 
基因编号
12505/960
 
同种型
IgG2b,kappa
 

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Mouse CD44 Antibody, Clone IM7, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a rat IgG2b, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (open histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2b, kappa Biotin isotype control antibody followed by SAV APC (solid line histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a rat IgG2b, kappa Biotin isotype control antibodyfollowed by SAV APC (open histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (open histogram).
(B) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Mouse CD44 Antibody, Clone IM7, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (open histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for APC-Conjugated

Figure 7. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD44 Antibody, Clone IM7, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60068AD, 60068AD.1
Lot #
All
Language
English
Catalog #
60068AZ, 60068AZ.1
Lot #
All
Language
English
Catalog #
60068BT.1, 60068BT
Lot #
All
Language
English
Catalog #
60068FI
Lot #
All
Language
English
Catalog #
60068PE, 60068PE.1
Lot #
All
Language
English
Catalog #
60068PS.1
Lot #
All
Language
English
Catalog #
60068.1, 60068
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068AD, 60068AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068AZ, 60068AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068BT.1, 60068BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068PE, 60068PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60068.1, 60068
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

Publications (1)

Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement. Freeman SA et al. Cell 2018 JAN

Abstract

Phagocytic receptors must diffuse laterally to become activated upon clustering by multivalent targets. Receptor diffusion, however, can be obstructed by transmembrane proteins (pickets") that are immobilized by interacting with the cortical cytoskeleton. The molecular identity of these pickets and their role in phagocytosis have not been defined. We used single-molecule tracking to study the interaction between Fcγ receptors and CD44 an abundant transmembrane protein capable of indirect association with F-actin hence likely to serve as a picket. CD44 tethers reversibly to formin-induced actin filaments curtailing receptor diffusion. Such linear filaments predominate in the trailing end of polarized macrophages where receptor mobility was minimal. Conversely receptors were most mobile at the leading edge where Arp2/3-driven actin branching predominates. CD44 binds hyaluronan anchoring a pericellular coat that also limits receptor displacement and obstructs access to phagocytic targets. Force must be applied to traverse the pericellular barrier enabling receptors to engage their targets.

更多信息

更多信息
Species Human, Mouse, Non-Human Primate, Other
Clone IM7
Gene Id 12505/960
Alternative Names ECMR III, gp85, H-CAM, Hermes, HUTCH-1, Ly24, Ly-24, Pgp-1
Isotype IgG2b, kappa
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