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抗小鼠CD117抗体，克隆ACK2

大鼠抗小鼠CD117（c-Kit）单克隆IgG2b抗体
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产品号 #60034_C

大鼠抗小鼠CD117（c-Kit）单克隆IgG2b抗体

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总览
实验数据
产品说明书及文档
应用领域
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总览

ACK2 单克隆抗体可识别 CD117（也称为 c-Kit），这是一种分子量约为 145 kDa 的 I 型跨膜受体，是 c-Kit 配体（即干细胞因子 / steel 因子）的受体。CD117 广泛表达于骨髓中的造血干细胞，包括多能干细胞、红系祖细胞以及 B 和 T 淋巴细胞前体细胞，同时也表达于肥大细胞表面。

CD117 属于酪氨酸激酶受体家族，结构上具有五个免疫球蛋白样的 C2 型结构域和一个胞质蛋白激酶结构域。当 c-Kit 配体与 CD117 结合后，可诱导其二聚化和自磷酸化，从而激活多个对造血干细胞的增殖和分化至关重要的细胞内信号通路。

CD117 的信号传导受到磷酸酶调控，以及受体的快速内吞和降解过程的调节。CD117 基因的突变与多种类型的肿瘤及“斑驳病”（piebald trait，一种常染色体显性遗传的色素异常）有关。

据报道，ACK2 抗体的结合可阻断 CD117 的功能。

亚型
一抗
 
靶抗原
CD117 (c-Kit)
 
别名
c-KIT，cKIT，干细胞因子受体（SCFR）
 
活性物种
小鼠
 
偶联
Alexa Fluor 488，APC，FITC，PE，未偶联的
 
宿主物种
大鼠
 
细胞类型
造血干/祖细胞
 
种属
小鼠
 
应用
流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀，Western印迹
 
研究领域
干细胞生物学
 
克隆
ACK2
 
基因编号
16590
 
同种型
IgG2b，kappa
 

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Data Figures

Data for PE-Conjugated

Figure 1. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 2. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.

Data for AlexaFluor® 488-Conjugated

Figure 3. Data for AlexaFluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488
Catalog #
60034AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, APC
Catalog #
60034AZ, 60034AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, PE
Catalog #
60034PE, 60034PE.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2
Catalog #
60034.1, 60034
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488
Catalog #
60034AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, APC
Catalog #
60034AZ, 60034AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, PE
Catalog #
60034PE, 60034PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD117 Antibody, Clone ACK2
Catalog #
60034.1, 60034
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Mouse
Clone ACK2
Gene Id 16590
Alternative Names c-KIT, cKIT, Stem cell factor receptor (SCFR)
Isotype IgG2b, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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