产品号 #60065_C
Mouse monoclonal IgM antibody against human, rat, rhesus TRA181
概述
This monoclonal antibody reacts with a carbohydrate epitope on TRA-1-81, a high-molecular-mass (> 200 kDa) glycoprotein expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells, as well as rhesus monkey and rat ES cell lines. No immunoreactivity is observed with mouse EC, EG, ES, or iPS cells. The epitope of the TRA-1-81 antibody is associated with a keratan-sulfated transmembrane protein identified as podocalyxin and is resistant to treatment with neuraminidase.
Subtype
Primary Antibodies
Target Antigen
TRA-1-81
Alternative Names
Podocalyxin
Reactive Species
Human, Rat, Rhesus
Conjugation
Alexa Fluor 488, APC, PE, Unconjugated
Host Species
Mouse
Cell Type
Pluripotent Stem Cells
Species
Human, Non-Human Primate, Rat
Application
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Stem Cell Biology
Clone
TRA-1-81
Gene ID
5420
Isotype
IgM, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, Alexa Fluor® 488 (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, Alexa Fluor® 488 (Catalog #60069AD) (solid line histogram).
Figure 2. Data for APC-Conjugated
Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, APC (filled histogram) or a mouse IgM, kappa APC isotype control antibody (solid line histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (Catalog #60069PE) (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE (filled histogram) or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, PE (solid line histogram).
Figure 4. Data for Unconjugated
(A) Flow cytometry analysis of human ES cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, followed by goat anti-mouse IgM, FITC (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 (Catalog #60069), followed by goat anti-mouse IgM, FITC (solid line histogram). (B) Human ES cells were cultured in mTeSR™1 on Corning® Matrigel®-coated glass slides, then fixed and labeled with Anti-Human TRA-1-81, Clone TRA-1-81, followed by goat anti-mouse IgG, FITC. Inset shows cells labeled with Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by goat anti-mouse IgG, FITC. (C) Flow cytometry analysis of human iPS cells labeled with Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgM, kappa Isotype Control Antibody, Clone MM-30, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram). (D) Western blot analysis of denatured/reduced cell lysates from human ES cells (lane 1) or HT1080 fibrosarcoma cells (lane 2) with Anti-Human TRA181 Antibody, Clone TRA-1-81.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, APC Catalog #
60065AZ Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE Catalog #
60065PE, 60065PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 Catalog #
60065 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, APC Catalog #
60065AZ Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81, PE Catalog #
60065PE, 60065PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 Catalog #
60065 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for PSC Quality Control
Resources and Publications
Educational Materials (3)
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