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抗人 CD9 抗体，克隆 HI9a

小鼠单克隆抗体，抗人、恒河猴、食蟹猴CD9，未偶联
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产品号 #100-0138_C

小鼠单克隆抗体，抗人、恒河猴、食蟹猴CD9，未偶联

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

HI9a 抗体与人 CD9（一种 24 kDa 单链 III 型跨膜糖蛋白）上的细胞外表位发生反应。CD9 广泛表达于单核细胞、发育中的 B 细胞、活化的 T 细胞、粒细胞、上皮细胞、内皮细胞和血小板（主要是 α 颗粒）的表面，也是细胞外囊泡的特征的标志物。

CD9 属于四跨蛋白（tetraspanin）家族，包含四个跨膜结构域、两个细胞外环以及短的细胞质 N 端和 C 端。它通过促进细胞粘附、迁移和信号转导，参与介导包括血管生成在内的多个细胞过程，并参与与受精、破骨细胞生成和肌生成有关的细胞融合过程。

亚型
一抗
 
靶抗原
CD9
 
别名
DRAP-24
 
活性物种
African Green Monkey，Baboon，Capuchin Monkey，Chimpanzee，Cynomolgus，人，Rhesus
 
偶联
未偶联的
 
宿主物种
小鼠
 
细胞类型
其它细胞系
 
应用
流式细胞术，Western印迹
 
研究领域
细胞外囊泡研究
 
克隆
HI9a
 
基因编号
928
 
同种型
IgG1，kappa
 

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Data Figures

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD9 Antibody, Clone HI9a and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD9 Antibody, Clone HI9a.

Figure 1. Analysis of Anti-Human CD9 Antibody, Clone HI9a with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) were isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column and analyzed by (A) western blot with Anti-Human CD9 Antibody, Clone HI9a. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin and then (B) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD9 Antibody, Clone HI9a, followed by a goat anti-mouse IgG1 antibody, APC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD9 Antibody, Clone HI9a
Catalog #
100-0138
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD9 Antibody, Clone HI9a
Catalog #
100-0138
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Clone HI9a
Gene Id 928
Alternative Names DRAP-24
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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