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EasySep™人细胞外囊泡（CD9）正选试剂盒

利用免疫磁珠正选技术快速简便地分离人细胞外囊泡（EV）
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产品号 #（选择产品）

产品号 #17894_C

利用免疫磁珠正选技术快速简便地分离人细胞外囊泡（EV）

产品优势

  • 快速、简单、无柱
  • 无需使用耗时且需要超速离心的方法分离细胞外囊泡
  • 获得高纯度、无生物液体成分污染的细胞外囊泡

产品组分包括

  • EasySep™人细胞外囊泡（CD9）正选试剂盒（产品号 #17894）
    • EasySep™人细胞外囊泡（CD9）正选抗体混合物，1 x 1 mL
    • EasySep™ Releasable RapidSpheres™ 50201磁珠，2 x 1 mL
    • 注意：分离的细胞外囊泡不从EasySep™Releable Rapidspheres™50201上解离
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

EasySep™人体细胞外囊泡（CD9）正选试剂盒通过免疫磁珠正选技术，从血浆/血清和细胞培养条件培养基中分离高纯度人体细胞外囊泡（EV）。细胞外囊泡被识别CD9的四聚体抗体复合物和磁珠标记。无需分离柱，使用EasySep™磁铁即可分离被标记的细胞外囊泡。不需要的生物液体组分通过简单倾倒弃去，而目的EV则保留在试管中。分离后的细胞外囊泡可用于下游应用，如DNA/RNA提取、Western blot或质谱分析。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001)
 
亚型
细胞分选试剂盒
 
细胞类型
其它细胞系
 
样本来源
其它细胞系
 
筛选方法
Positive
 
应用
细胞分选，细胞外囊泡分选
 
品牌
EasySep
 
研究领域
细胞外囊泡研究
 

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Data Figures

Positive isolation of EVs with the CD9 tetraspanin marker from human plasma and MSC-conditioned medium.

Figure 1. Typical Western Blot Analyses of EVs Isolated from Human plasma and Mesenchymal Stromal Cell (MSC)-Conditioned Medium

The Western blot analyses in the above examples show positive isolation of EVs with the CD9 tetraspanin marker from (A) human plasma and (B) MSC-conditioned medium. Western blots were run under non-reducing conditions.

Figure 2. Tetraspanin Protein Expression and Recovery of EVs Using EasySep™ Human Pan/CD9/CD63/CD81 Extracellular Vesicle Positive Selection Kits

EVs were isolated from plasma using either EasySep™ Human Extracellular Vesicle Positive Selection Kits or differential ultracentrifugation (2 x 70 min, 100,000 xg) and isolated fractions were analyzed by western blot for tetraspanin protein expression.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Extracellular Vesicle (CD9) Positive Selection Kit
Catalog #
17894
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001)
Sample Source Other
Selection Method Positive
标记抗体
  1. 细胞外囊泡人CD9/CD63/CD81抗体组合
    细胞外囊泡人CD9/CD63/CD81抗体组合

    使用 CD9、CD63 和 CD81 标记物检测细胞外囊泡的抗体试剂盒

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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