产品号 #60114_C
小鼠单克隆IgG1抗体,抗人、恒河猴、食蟹猴CD41
HIP8抗体识别人CD41的~125 kDa GPIIbα亚基,CD41是一种异二聚体整合素,主要在血小板、巨核细胞、单核细胞和急性巨核母细胞白血病细胞上表达。GPIIbα亚基通过单二硫键与较小的~25 kDa亚基(GPIIbβ)相连。CD41的三种异构体是由选择性剪接产生的,其中一种只在癌细胞中表达。CD41以钙依赖的方式与CD61(整合素b3)非共价结合,形成CD41/CD61(或GPIIb/IIIa)复合物,该复合物已被鉴定为多种配体(包括纤维蛋白原、纤连蛋白、玻连蛋白和凝血酶)的受体。与CD41/CD61复合物的结合涉及配体中的RGD三肽序列,而在整合素介导的应答中,配体占据和配体介导的受体聚集都具有重要功能。据报导,HIP8抗体通过数种化合物阻断血小板聚集并抑制血小板活化。
亚型
一抗
靶抗原
CD41
别名
GPIIb,整合素α2b,整合素αIIb,ITGA2B,血小板糖蛋白IIb
活性物种
Baboon,Capuchin Monkey,Chimpanzee,Cynomolgus,人,Pig,Rhesus
偶联
APC,FITC,PE,未偶联的
宿主物种
小鼠
细胞类型
巨核细胞,血小板
种属
人,非人灵长类,其它细胞系
应用
CyTOF,流式细胞术,功能学筛选,免疫荧光,免疫组化,中和及阻断
研究领域
免疫
克隆
HIP8
基因编号
3674
同种型
IgG1,kappa
Figure 1. Data for Unconjugated
Flow cytometry analysis of human platelets labeled with Anti-Human CD41 Antibody, Clone HIP8, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).
Figure 2. Data for APC-Conjugated
Flow cytometry analysis of human platelets labeled with Anti-Human CD41 Antibody, Clone HIP8, APC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ, solid line histogram).
Figure 3. Data for FITC-Conjugated
Flow cytometry analysis of human platelets labeled with Anti-Human CD41 Antibody, Clone HIP8, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI, solid line histogram).
Figure 4. Data for PE-Conjugated
Flow cytometry analysis of human platelets labeled with Anti-Human CD41 Antibody, Clone HIP8, PE (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE, solid line histogram).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Species | Human, Non-Human Primate, Other |
---|---|
Clone | HIP8 |
Gene Id | 3674 |
Alternative Names | GPIIb, Integrin alpha 2b, Integrin alpha llb, ITGA2B, Platelet glycoprotein IIb |
Isotype | IgG1, kappa |
用于分离单个核细胞的密度梯度离心液
红细胞裂解缓冲液
小鼠单克隆IgG1, kappa同型对照抗体
小鼠单克隆IgG1, kappa同型对照抗体
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