产品号 #100-0285_C
Mouse monoclonal IgG2a antibody against human CD3
概述
The OKT3 antibody reacts with an extracellular conformational epitope on the ~20 kDa CD3ε subunit of the human T cell receptor (TCR)/CD3 complex, which is expressed on the surface of ~95% of mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. CD3 is a dimer assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. CD3 associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating T cell activation and the expression of the TCR on the cell surface. Mutations in CD3 have been associated with disorders of the immune system, including severe combined immunodeficiency (SCID). The OKT3 antibody recognizes an epitope that is expressed when the CD3ε subunit associates with either CD3γ or CD3δ, and binding of the antibody can induce T cell activation. Because of its immunosuppressive properties in vivo, the OKT3 antibody has proven useful in treating allograft rejection. Clone OKT3 is widely used for phenotyping human T cells, and it has been observed to block the binding of anti-CD3 antibody clones SK7 and UCHT1.
Subtype
Primary Antibodies
Target Antigen
CD3
Alternative Names
CD3 epsilon, CD3ε, Leu-4, T3
Reactive Species
Human
Conjugation
APC, FITC, Unconjugated
Host Species
Mouse
Cell Type
T Cells
Application
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blotting
Area of Interest
Immunology
Clone
OKT3
Gene ID
916
Isotype
IgG2a, kappa
Data Figures
Figure 1. Data for Anti-Human CD3 Antibody, Clone OKT3, APC
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone OKT3, APC (filled histogram) or a mouse IgG2a, kappa APC isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.
Figure 2. Data for Anti-Human CD3 Antibody, Clone OKT3, FITC
Flow cytometry analysis of human PBMCs labeled with Anti-Human CD3 Antibody, Clone OKT3, FITC (filled histogram) or a mouse IgG2a, kappa FITC isotype control antibody (solid line histogram). Viable lymphocytes were gated for analysis.
Figure 3. Data for Anti-Human CD3 Antibody, Clone OKT3, Unconjugated
Flow cytometry analysis of human PBMCs labeled with Anti-Human CD3 Antibody, Clone OKT3, followed by a goat anti-mouse IgG2a antibody, PE (filled histogram) or a mouse IgG2a, kappa isotype control antibody, followed by a goat anti-mouse IgG2a antibody, PE (solid line histogram). Viable lymphocytes were gated for analysis.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone OKT3, APC Catalog #
100-0289, 100-0288 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone OKT3, FITC Catalog #
100-0286, 100-0285 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone OKT3 Catalog #
100-0287 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone OKT3, APC Catalog #
100-0289, 100-0288 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone OKT3, FITC Catalog #
100-0286, 100-0285 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Dendritic Cell Research
Workflow Stages for Natural Killer Cell Research
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