产品号 #60083_C
Mouse monoclonal IgG1 antibody against human, chimpanzee CD22
概述
The HIB22 antibody reacts with CD22 (Siglec-2), a type I transmembrane glycoprotein expressed in the cytoplasm of pro-B and pre-B cells and on the surface of mature and activated B cells and B cell lymphomas, but not on plasma cells. CD22 functions as an inhibitory receptor for B cell receptor signaling and plays roles in modulating the activation threshold of B cells and controlling CD40 signaling. CD22 comprises two isoforms generated by alternative splicing: CD22α (~130 kDa) and CD22β (~140 kDa). Monomeric CD22β is the most abundant isoform but an αβ heterodimer is also expressed. CD22 is a member of the sialoadhesion subgroup within the immunoglobulin superfamily, with an N-terminal V-type Ig domain followed by six C2-type Ig domains. Binding of the HIB22 antibody reportedly requires the presence of the two N-terminal-most domains. Both the cytoplasmic and surface-bound forms of CD22 are recognized by the HIB22 antibody and its binding does not inhibit CD22-mediated cyto-adhesion.
Subtype
Primary Antibodies
Target Antigen
CD22
Alternative Names
B-lymphocyte cell adhesion molecule, BLCAM, Leu-14, Lyb8, Sialic acid-binding Ig-like lectin 2, Siglec-2
Reactive Species
Chimpanzee, Human
Conjugation
APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells
Species
Human, Non-Human Primate
Application
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
HIB22
Gene ID
933
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).
Figure 2. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).
Figure 3. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).
Figure 4. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD22 Antibody, Clone HIB22, APC Catalog #
60083AZ Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD22 Antibody, Clone HIB22 Catalog #
60083 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD22 Antibody, Clone HIB22, APC Catalog #
60083AZ Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD22 Antibody, Clone HIB22 Catalog #
60083 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Chimerism Analysis
Resources and Publications
Educational Materials (3)
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