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抗人CD22 抗体，克隆HIB22

小鼠单克隆IgG1抗体，抗人、黑猩猩CD22
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产品号 #60083_C

小鼠单克隆IgG1抗体，抗人、黑猩猩CD22

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总览
实验数据
产品说明书及文档
应用领域
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总览

HIB22抗体识别CD22 （Siglec-2），CD22是一种I型跨膜糖蛋白，表达于B祖细胞和前体细胞的细胞质以及成熟和活化的B细胞和B细胞淋巴瘤的表面，但不表达于浆细胞。CD22作为B细胞受体信号传导的抑制受体，在调节B细胞的激活阈值和控制CD40信号传导中发挥作用。CD22包括两种选择性剪接产生的异构体：CD22α (~130 kDa)和CD22β (~140 kDa)。单体CD22β是最丰富的亚型，但αβ异源二聚体也有表达。CD22是免疫球蛋白超家族中唾液粘附亚群的成员，具有N端V-type Ig结构域和6个C2-type Ig结构域。据报道，HIB22抗体的结合需要两个最 N 端结构域的存在。CD22的细胞质和表面结合形式都能被HIB22抗体识别，并且它的结合不会抑制CD22介导的细胞粘附。

亚型
一抗
 
靶抗原
CD22
 
别名
B细胞黏附分子, BLCAM，Leu-14，Lyb8，Sialic acid-binding Ig-like lectin 2，Siglec-2
 
活性物种
Chimpanzee，人
 
偶联
APC，FITC，PE，未偶联的
 
宿主物种
小鼠
 
细胞类型
B 细胞
 
种属
人，非人灵长类
 
应用
ELISAs，流式细胞术，功能学筛选，免疫细胞化学，免疫荧光，免疫组化，免疫沉淀
 
研究领域
免疫
 
克隆
HIB22
 
基因编号
933
 
同种型
IgG1，kappa
 

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Data Figures

Data for APC-Conjugated

Figure 1. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (solid line histogram).

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (solid line histogram).

Data for PE-Conjugated

Figure 3. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood B Cell Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, PE. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram). (C) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD19 Positive Selection Kit and labeled with Anti-Human CD22 Antibody, Clone HIB22, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa PE isotype control antibody is shown (solid line histogram).

Data for Unconjugated

Figure 4. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD22 Antibody, Clone HIB22, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD22 Antibody, Clone HIB22, APC
Catalog #
60083AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD22 Antibody, Clone HIB22
Catalog #
60083
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD22 Antibody, Clone HIB22, APC
Catalog #
60083AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD22 Antibody, Clone HIB22
Catalog #
60083
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (3)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

更多信息

更多信息
Species Human, Non-Human Primate
Clone HIB22
Gene Id 933
Alternative Names B-lymphocyte cell adhesion molecule, BLCAM, Leu-14, Lyb8, Sialic acid-binding Ig-like lectin 2, Siglec-2
Isotype IgG1, kappa
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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