EasySep™ Human Th17 Cell Enrichment Kit II

Immunomagnetic depletion of human TCRab+ cells

产品优势


  • Fast, easy-to-use, and column-free procedure

  • Untouched, viable cells

  • Efficiently deplete human T cell receptor alpha/beta+ (TCRαß+) cells from leukapheresis samples or expanded TCRαß knockout cell culture samples by immunomagnetic positive selection, with the EasySep™ Human TCR Alpha/Beta Depletion Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

    This straightforward, optimized EasySep™ procedure involves labeling cells with antibody complexes recognizing TCRαß and magnetic particles. Labeled cells are separated from untouched cells using an EasySep™ magnet and by simply pouring or pipetting off the unlabeled cells. The TCRαß+ cells remain in the tube. Following magnetic cell isolation in as little as 13 minutes, desired cells are ready for downstream applications such as flow cytometry, culture, or cryopreservation.

    For large-scale depletion of human T cell receptor alpha/beta+ (TCRαß+) cells from leukapheresis samples, see the large-format (1x10^10 cells) kit (Catalog #100-1660).

    Learn more about how immunomagnetic EasySep™ technology works. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.

    Data Figures

    The content of TCRab+ cells before and after the cell isolation procedure are 19.1% and 0.1%, respectively.

    Figure 1. Typical EasySep™ Human TCR Alpha/Beta Depletion Profile

    In the above example, the frequencies of CD3+TCRαß+ cells in the starting and depleted fractions are 19.1% and 0.1%, respectively.

    Residual TCRαβ+ Cells Can Be Removed from Expanded TRAC Knockout Cell Populations Using EasySep™ Human TCRαβ Depletion Kit

    Figure 2. EasySep™ Human TCRαβ Depletion Kit Can Be Used to Remove Residual TCRαβ+ Cells from Expanded TRAC Knockout Cell Populations After Genome Editing of Human Primary T Cells Using CRISPR-Cas9

    Gene-edited TCRαβ knockout T cells were expanded using ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 - 10 days, harvested, and resuspended in EasySep™ Buffer (Catalog # 20144) at 5 x 10⁷ cells/mL. Following a 13-minute EasySep™ depletion protocol using EasySep™ Human TCRαβ Depletion Kit (Catalog #17847), flow cytometry was performed to assess the depletion of TCRαβ+ cells. Representative data plots are shown for the frequency of TCRαβ cells in the expanded TRAC knockout cell population before and after EasySep™ depletion.

    Protocols and Documentation

    Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

    Document Type
    Product Name
    Catalog #
    Lot #
    Language
    Catalog #
    100-1660
    Lot #
    All
    Language
    English
    Catalog #
    17847
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet
    Catalog #
    100-1660
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 1
    Catalog #
    17847
    Lot #
    All
    Language
    English
    Document Type
    Safety Data Sheet 2
    Catalog #
    17847
    Lot #
    All
    Language
    English

    Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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