The EasySep™ Human Whole Blood CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh whole blood or freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.
This product can be used instead of the EasySep™ Human Whole Blood CD34 Positive Selection Kit (Catalog #18086).
If isolating CD34+ cells from fresh cord blood, we recommend using the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896). Alternatively, if isolating CD34+ cells from any other sample type, the EasySep™ Human CD34 Positive Selection Kit II (Catalog #17856) should be used.
Magnet Compatibility <div>
• "The Big Easy" EasySep™ Magnet (Catalog #18001)
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• RoboSep™-S (Catalog #21000)
Subtype Cell Isolation Kits
Cell Type Hematopoietic Stem and Progenitor Cells
Species Human
Sample Source Buffy Coat, Whole Blood
Selection Method Positive
Application Cell Isolation
Brand EasySep, RoboSep
Area of Interest Chimerism, Immunology, Stem Cell Biology
Data Figures
Starting with whole blood, the CD34+ cell content of the isolated fraction is typically 90.4 ± 7.0% (gated on CD45+ cells; mean ± SD using RoboSep™-S). In the above example, the purities of the start and final isolated fractions are 0.06% and 95.31%, respectively. NOTE: RBCs were removed by lysis prior to flow cytometry.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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