EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

产品号 #17881_C

Immunomagnetic positive selection cell isolation kit

产品优势


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

产品组分包括

  • EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit (Catalog #17881)
    • EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit with Filter Tips (Catalog #17881RF)
    • EasySep™ Human Whole Blood CD15 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol

概述

The EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit is designed to isolate highly purified CD15+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD15 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD15 antigen is expressed on granulocytes and monocytes.

This product replaces the EasySep™ Human Whole Blood CD15 Positive Selection Kit (Catalog #18681) and EasySep™ HLA Whole Blood CD15 Positive Selection Kit (Catalog #18681HLA), for even faster cell isolations.

Magnet Compatibility
<div> • “The Big Easy” EasySep™ Magnet (Catalog #18001) </div> <div> • EasyEights™ EasySep™ Magnet (Catalog #18103) </div> <div> • RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
 
Cell Type
Myeloid Cells
 
Species
Human
 
Sample Source
PMNC, Whole Blood
 
Selection Method
Positive
 
Application
Cell Isolation
 
Brand
EasySep, RoboSep
 
Area of Interest
Chimerism, HLA, Immunology
 

Data Figures

Starting with human whole blood, the CD15+ cell content of the isolated fraction is typically 99.2 ± 1.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 57.6% and 99.9%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 2
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Safety Data Sheet 3
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17881
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English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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