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CryoStor® CS10

Animal component-free, defined cryopreservation medium with 10% DMSO
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产品号 #07930_C

Animal component-free, defined cryopreservation medium with 10% DMSO

产品优势


  • Ready-to-use

  • Serum-free and protein-free

  • Animal component-free

  • cGMP manufactured with USP grade/highest-quality components

  • FDA master file

  • Sterility, endotoxin, and cell-based quality control testing

main product photo
CryoStor® CS10
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

Maximize post-thaw cell recovery and viability following cryopreservation at very low temperatures (-70°C to -196°C) with ready-to-use CryoStor® CS10 medium. Serum-free, animal component-free, and cGMP-manufactured, this defined medium provides a safe, protective environment that is recommended for the cryopreservation of a variety of sensitive cell and sample types, including myeloma cell lines, human pluripotent stem cells, blood-derived cells, and more. Available in a variety of convenient formats, CryoStor® CS10 is formulated with USP-grade components to minimize variability and contains 10% DMSO.

Contains
• 10% dimethyl sulfoxide (DMSO) <br> • Other ingredients
 
Cell Type
B Cells, CHO Cells, Hematopoietic Stem and Progenitor Cells, Hybridomas, Intestinal Cells, Macrophages, Mesenchymal Stem and Progenitor Cells, Monocytes, Myeloma, NK Cells, Other, Pluripotent Stem Cells, T Cells
 
Species
Human, Mouse, Non-Human Primate, Other, Rat
 
Application
Cryopreservation
 
Brand
CryoStor
 
Area of Interest
Cord Blood Banking, Epithelial Cell Biology, Immunology, Stem Cell Biology
 
Formulation Category
Animal Component-Free, Serum-Free
 

Data Figures

Figure 1. Immune Cells Cryopreserved in CryoStor®CS10 Show Reproducibly High Post-Thaw Cell Viability

CryoStor®CS10 effectively mitigates temperature-induced molecular cell stress responses to maximize post-thaw viability and recovery for a variety of immune cell types, including T cells (data not shown) and B cells. Here, human B cells from 6 different donors cryopreserved in CryoStor®CS10 show reproducibly high viability after thawing, as measured by Propidium Iodide staining (ranging from 94.3 - 97.9%).

Figure 2. Immune Cells Cryopreserved in CryoStor®CS10 Retain Functionality Post-Thaw

(A) Human peripheral blood Pan-T cells cryopreserved in CryoStor®CS10 were thawed and cultured with or without the addition of T cell activating factors. Cells from Donors 1-3 were cultured in RPMI Medium supplemented with 10% FBS, with (activated) or without (control) 40 ng/mL PMA and 1 ug/mL Ionomycin for 24 hours. Cells from Donors 4-5 were cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981), with (activated) or without (control) ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) for 48 hours. Supernatants were collected from the cultures, and concentrations of secreted cytokines were determined using the Human IL-2 ELISA Kit (Catalog #02006). Activation by either PMA and Ionomycin or ImmunoCult™ Human CD3/CD28 T Cell Activator led to increased secretion of IL-2 compared to unstimulated control cultures. (B) Human B cells (Donors 6 - 11) cryopreserved in CryoStor®CS10 were thawed and activated with 1 µg/mL CD40 and 100 ng/mL IL-21 for 7 days. Supernatants were collected from the cultures and immunoglobulin G (IgG) production was measured using the Human IgG ELISA Antibody Pair Kit (Catalog #01994). Compared to unstimulated control cultures, B cell activation led to increased IgG​ ​secretion.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Stability Memo
Product Name
CryoStor® CS10
Catalog #
100-1061
Lot #
24166 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
CryoStor® CS10
Catalog #
100-1061, 07959, 07931, 07955, 07940, 07930, 07952
Lot #
For Catalog 07959, 07931, 07955, 07940, 07930, and 07952: All lots. For Catalog 100-1061: 24167 or higher
Language
English
Document Type
Product Information Sheet 1
Product Name
CryoStor® CS10
Catalog #
100-1061
Lot #
24166 or lower
Language
English
Document Type
Safety Data Sheet
Product Name
CryoStor® CS10
Catalog #
100-1061, 07959, 07931, 07955, 07940, 07930
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (21)

hPSC-Derived Cell Therapy Workflow
Brochure
hPSC-Derived Cell Therapy Workflow
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
T Cell Reagents for Your Cellular Therapy Research
Brochure
T Cell Reagents for Your Cellular Therapy Research
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Cryopreservation Media for Stem Cell Research
Brochure
Cryopreservation Media for Stem Cell Research
How to Cryopreserve Neurospheres
Protocol
How to Cryopreserve Neurospheres
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
How to Count Intestinal Crypts for Mouse Organoid Cultures
1:09
Video
How to Count Intestinal Crypts for Mouse Organoid Cultures
How to Thaw Frozen Human Primary Cells
6:05
Video
How to Thaw Frozen Human Primary Cells
Cryopreserve Human Peripheral Blood Mononuclear Cells with CryoStor® CS10
3:41
Video
Cryopreserve Human Peripheral Blood Mononuclear Cells with CryoStor® CS10
High-Quality Mobilized Leukopaks to Enhance Your Cell and Gene Therapy Research and Development
44:31
Webinar
High-Quality Mobilized Leukopaks to Enhance Your Cell and Gene Therapy Research and Development
Modeling the Human Alveolar Region with Organoids
20:53
Webinar
Modeling the Human Alveolar Region with Organoids
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
42:10
Webinar
Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenanc...
Applications and Analysis of Organoids
47:16
Webinar
Applications and Analysis of Organoids
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
16:31
Webinar
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
Combining CAR T Cells with STING Agonists to Fight Solid Tumors
52:59
Webinar
Combining CAR T Cells with STING Agonists to Fight Solid Tumors
Human T Cell Expansion Course
On-Demand Training
Human T Cell Expansion Course
Human Immune Cell Isolation Course
On-Demand Training
Human Immune Cell Isolation Course

Publications (59)

The immune cell landscape in kidneys of patients with lupus nephritis. A. Arazi et al. Nature immunology 2019

Abstract

Lupus nephritis is a potentially fatal autoimmune disease for which the current treatment is ineffective and often toxic. To develop mechanistic hypotheses of disease, we analyzed kidney samples from patients with lupus nephritis and from healthy control subjects using single-cell RNA sequencing. Our analysis revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid cells, T cells, natural killer cells and B cells that demonstrated both pro-inflammatory responses and inflammation-resolving responses. We found evidence of local activation of B cells correlated with an age-associated B-cell signature and evidence of progressive stages of monocyte differentiation within the kidney. A clear interferon response was observed in most cells. Two chemokine receptors, CXCR4 and CX3CR1, were broadly expressed, implying a potentially central role in cell trafficking. Gene expression of immune cells in urine and kidney was highly correlated, which would suggest that urine might serve as a surrogate for kidney biopsies.
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Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses. U. Rajamani et al. Cell stem cell 2018 MAY

Abstract

The hypothalamus contains neurons that integrate hunger and satiety endocrine signals from the periphery and are implicated in the pathophysiology of obesity. The limited availability of human hypothalamic neurons hampers our understanding of obesity disease mechanisms. To address this, we generated human induced pluripotent stem cells (hiPSCs) from multiple normal body mass index (BMI; BMI ≤ 25) subjects and super-obese (OBS) donors (BMI ≥ 50) with polygenic coding variants in obesity-associated genes. We developed a method to reliably differentiate hiPSCs into hypothalamic-like neurons (iHTNs) capable of secreting orexigenic and anorexigenic neuropeptides. Transcriptomic profiling revealed that, although iHTNs maintain a fetal identity, they respond appropriately to metabolic hormones ghrelin and leptin. Notably, OBS iHTNs retained disease signatures and phenotypes of high BMI, exhibiting dysregulated respiratory function, ghrelin-leptin signaling, axonal guidance, glutamate receptors, and endoplasmic reticulum (ER) stress pathways. Thus, human iHTNs provide a powerful platform to study obesity and gene-environment interactions.
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Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells. O. V. Halaidych et al. Stem cell reports 2018 MAY

Abstract

Several studies have reported endothelial cell (EC) derivation from human induced pluripotent stem cells (hiPSCs). However, few have explored their functional properties in depth with respect to line-to-line and batch-to-batch variability and how they relate to primary ECs. We therefore carried out accurate characterization of hiPSC-derived ECs (hiPSC-ECs) from multiple (non-integrating) hiPSC lines and compared them with primary ECs in various functional assays, which included barrier function using real-time impedance spectroscopy with an integrated assay of electric wound healing, endothelia-leukocyte interaction under physiological flow to mimic inflammation and angiogenic responses in in vitro and in vivo assays. Overall, we found many similarities but also some important differences between hiPSC-derived and primary ECs. Assessment of vasculogenic responses in vivo showed little difference between primary ECs and hiPSC-ECs with regard to functional blood vessel formation, which may be important in future regenerative medicine applications requiring vascularization.
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CRYOSTOR PRODUCTS MEET USP STERILITY AND USP ENDOTOXIN TESTING STANDARDS, AND ARE MANUFACTURED UNDER CGMP.
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