产品号 #100-1545_C
For detection and distinction of viable and non-viable cells, using two fluorogenic dyes
概述
Viability Assay Kit, Green/Red Fluorescence provides a convenient and robust method to determine cell viability through the use of two fluorogenic dyes (Calcein AM and Propidium Iodide) that permit the simultaneous detection and distinction of viable and non-viable cells. As a fluorogenic dye, Calcein AM is initially non-fluorescent. After its passive entry into cells, intracellular esterases which are present only in viable cells, hydrolyze Calcein AM into Calcein, a bright green fluorescent molecule (Bratosin et al.). The intensity of the green fluorescence is proportional to the amount of esterase activity and thus can correlate to the number of viable cells. Propidium Iodide is the second fluorogenic dye; however, unlike Calcein AM, can only cross the compromised membranes of dead cells. Once inside dead cells, Propidium Iodide fluoresces red upon its binding to DNA. The dyes in this kit are well suited for use with fluorescence microscopes or fluorescence microplate readers that are capable of detecting in the FITC (for Calcein) and TRITC (for Propidium Iodide) channels.
Use Cell Viability Assay Kit, Green/Red Fluorescence to detect and quantify cell proliferation in adherent or suspension cultures or incorporate it as a useful readout in your in vitro cytotoxicity assays.
Application
Characterization
Data Figures
Figure 1. Live or Dead Cell Viability Assay Kit Selectively Labels Live and Dead Cells in Fluorescent Green and Red
(A) Live HEK293 cells (top row) and dead HEK293 cells (bottom row) were stained separately using Cell Viability Assay Kit and then imaged using a fluorescence microscope. Live cells emit fluorescent green, while dead cells with compromised cell membranes emit fluorescent red. (B) Live HEK293 cells were treated with staurosporine, a cytotoxic agent, for 24 hours. Cells were then stained with Cell Viability Assay Kit and imaged using a fluorescence microscope. The merged image shows a mixed population of live and dead cells.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Cell Viability Assay Kit, Green/Red Fluorescence Catalog #
100-1545 Lot #
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Safety Data Sheet 1 Product Name
Cell Viability Assay Kit, Green/Red Fluorescence Catalog #
100-1545 Lot #
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Cell Viability Assay Kit, Green/Red Fluorescence Catalog #
100-1545 Lot #
All Language
English Document Type
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Cell Viability Assay Kit, Green/Red Fluorescence Catalog #
100-1545 Lot #
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Cell Viability Assay Kit, Green/Red Fluorescence Catalog #
100-1545 Lot #
All Language
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