产品号 #60060_C
Mouse monoclonal IgM antibody against human, mouse, rat SSEA-1 (CD15)
概述
The MC-480 antibody reacts with a terminal carbohydrate epitope, stage-specific embryonic antigen-1 (SSEA-1), which is expressed on a large-molecular-mass (> 200 kDa) glycoprotein on the surface of early mouse embryos, mouse embryonal carcinoma (EC), embryonic stem (ES) cells and mouse and human embryonic germ (EG) cells. SSEA-1 is not expressed on undifferentiated human EC, ES or induced pluripotent stem (iPS) cells, or rhesus monkey ES cell lines. Its expression on mouse ES cells is decreased upon differentiation, whereas in humans, expression is upregulated during differentiation. SSEA-1 is also found on adult human granulocytes and monocytes, where it is denoted CD15, and the MC-480 antibody recognizes the CD15 marker on these cell types. It has been reported that SSEA-1 has roles in cell adhesion and migration, and regulation of cell differentiation.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ HLA Whole Blood CD15 Positive Selection Kit (Catalog #18681HLA; partial blocking may be observed), and for labeling human ES and iPS cells grown in TeSR™-E8™ (Catalog #05940), mTeSR™1 (Catalog #85850) and TeSR™2 (Catalog #05860).
Subtype
Primary Antibodies
Target Antigen
SSEA-1 (CD15)
Alternative Names
3-FAL, CD15, Lewis X, SSEA1, Stage-specific embryonic antigen 1, X-hapten
Reactive Species
Human, Mouse, Rat
Conjugation
Alexa Fluor 488, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Pluripotent Stem Cells
Species
Human, Mouse, Rat
Application
Cell Isolation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Stem Cell Biology
Clone
MC-480
Gene ID
14345
Isotype
IgM, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
OCT4 as a target of miR-34a stimulates p63 but inhibits p53 to promote human cell transformation Cell death & disease 2014 JAN
Abstract
Human cell transformation is a key step for oncogenic development, which involves multiple pathways; however, the mechanism remains unclear. To test our hypothesis whether cell oncogenic transformation shares some mechanisms with the process of reprogramming non-stem cells to induced pluripotent stem cells (iPSC), we studied the relationship among the key factors for promoting or inhibiting iPSC in radiation-transformed human epithelial cell lines derived from different tissues (lung, breast and colon). We unexpectedly found that p63 and OCT4 were highly expressed (accompanied by low expressed p53 and miR-34a) in all transformed cell lines examined when compared with their non-transformed counterparts. We further elucidated the relationship of these factors: the 3p strand of miR-34a directly targeted OCT4 by binding to the 3′ untranslated region (3′-UTR) of OCT4 and, OCT4, in turn, stimulated p63 but inhibited p53 expression by binding to a specific region of the p63 or p53 promoter. Moreover, we revealed that the effects of OCT4 on promoting cell oncogenic transformation were by affecting p63 and p53. These results support that a positive loop exists in human cells: OCT4 upregulation as a consequence of inhibition of miR-34a, promotes p63 but suppresses p53 expression, which further stimulates OCT4 upregulation by downregulating miR-34a. This functional loop contributes significantly to cell transformation and, most likely, also to the iPSC process.
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
