产品号 #60031_C
Rat monoclonal IgG2a antibody against mouse Ly-6G
概述
The 1A8 antibody reacts with Ly-6G, a 21 - 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery, Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).
Subtype
Primary Antibodies
Target Antigen
Ly-6G
Alternative Names
Gr-1, GR1, Ly6G
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, Pacific Blue, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Mouse
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
1A8
Gene ID
546644
Isotype
IgG2a, kappa
Data Figures
Figure 1. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).
Figure 2. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).
Figure 3. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). (B) Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse uterine tissue labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by anti-mouse, HRP. DAB substrate was used for visualization.
Figure 4. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).
Figure 5. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).
Figure 6. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
Figure 7. Data for PB-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, APC Catalog #
60031AZ, 60031AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin Catalog #
60031BT.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC Catalog #
60031FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ Catalog #
60031PB, 60031PB.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PE Catalog #
60031PE, 60031PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 Catalog #
60031PS.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8 Catalog #
60031 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, APC Catalog #
60031AZ, 60031AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin Catalog #
60031BT.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC Catalog #
60031FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ Catalog #
60031PB, 60031PB.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PE Catalog #
60031PE, 60031PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 Catalog #
60031PS.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse Ly-6G Antibody, Clone 1A8 Catalog #
60031 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Dendritic Cell Research
Workflow Stages for Granulocyte Research
Resources and Publications
Educational Materials (4)
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Legal Statement: Pacific Blue™ is a trademark of Life Technologies Corporation. Antibodies conjugated to Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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