产品号 #60023_C
Rat monoclonal IgG2a antibody against mouse, toad CD8a
概述
The 53-6.7 antibody reacts with murine CD8a, a 32 - 34 kDa type I transmembrane glycoprotein which is a subunit of CD8. CD8 is a disulfide-bonded dimer, found either as a heterodimer of CD8a (α) and CD8b (β) subunits (i.e., αβ) or a homodimer (αα). CD8 acts as a co-receptor to the T cell receptor (TCR) during T cell activation by binding MHC Class I molecules presented by an antigen-presenting cell. It functions to strengthen the association between the TCR and MHC I-antigen complex and to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases such as Lck. The CD8a chain binds to the alpha-3 domain of class I MHC molecules. CD8 is expressed in the αβ form by a majority of thymocytes and a subset of mature peripheral blood T cells (T cytotoxic cells), and in the ββ form by γδ T cells, a subset of intestinal intraepithelial lymphocytes and dendritic cells. The 53-6.7 antibody reportedly blocks antigen presentation via MHC class I and inhibits IL-2-dependent T cell responses.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD8+ T Cell Isolation Kit (Catalog #19853) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).
Subtype
Primary Antibodies
Target Antigen
CD8a
Alternative Names
Ly-2, Lyt2, T8
Reactive Species
Mouse, Toad
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
T Cells, T Cells, CD8+
Species
Mouse, Other
Application
Cell Isolation, CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
53-6.7
Gene ID
12525
Isotype
IgG2a, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
