产品号 #60068_C
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44 (tissue non-specific alkaline phosphatase)
概述
The IM7 antibody reacts with CD44 (Ly-24), an ~80 - 95 kDa type 1 transmembrane glycoprotein involved in cell-cell and cell-matrix interactions. CD44 is expressed on the surface of many cells, including leukocytes and hepatocytes, as well as endothelial, epithelial, and mesenchymal cells. Expression levels increase upon activation of T and B cells, and memory cells exhibit a CD44high phenotype. CD44 binds many ligands, including hyaluronic acid, collagen, fibronectin, growth factors, and metalloproteinases, thus modulating processes such as lymphocyte activation, recirculation and homing, leukocyte rolling and aggregation, hematopoiesis, and tumor metastasis. Numerous disorders are associated with altered expression or dysfunction of CD44. Many CD44 isoforms have been identified, with alternative splicing, differential N- and O- glycosylation, and sulfation mediating the functional role(s) played by the protein in a specific cell. The IM7 monoclonal antibody reacts with an extracellular epitope found on all isoforms of CD44 and both murine allotypes.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852), EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit (Catalog #18765), and EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555).
Subtype
Primary Antibodies
Target Antigen
CD44
Alternative Names
ECMR III, gp85, H-CAM, Hermes, HUTCH-1, Ly24, Ly-24, Pgp-1
Reactive Species
Baboon, Cat, Chimpanzee, Cow, Cynomolgus, Dog, Horse, Human, Mouse, Pig, Rhesus, Squirrel Monkey
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Mammary Cells, T Cells
Species
Human, Mouse, Non-Human Primate, Other
Application
Cell Isolation, CyTOF, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Epithelial Cell Biology, Immunology, Stem Cell Biology
Clone
IM7
Gene ID
12505/960
Isotype
IgG2b, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
Transmembrane Pickets Connect Cyto- and Pericellular Skeletons Forming Barriers to Receptor Engagement. Cell 2018 JAN
Abstract
Phagocytic receptors must diffuse laterally to become activated upon clustering by multivalent targets. Receptor diffusion, however, can be obstructed by transmembrane proteins (pickets") that are immobilized by interacting with the cortical cytoskeleton. The molecular identity of these pickets and their role in phagocytosis have not been defined. We used single-molecule tracking to study the interaction between Fcγ receptors and CD44 an abundant transmembrane protein capable of indirect association with F-actin hence likely to serve as a picket. CD44 tethers reversibly to formin-induced actin filaments curtailing receptor diffusion. Such linear filaments predominate in the trailing end of polarized macrophages where receptor mobility was minimal. Conversely receptors were most mobile at the leading edge where Arp2/3-driven actin branching predominates. CD44 binds hyaluronan anchoring a pericellular coat that also limits receptor displacement and obstructs access to phagocytic targets. Force must be applied to traverse the pericellular barrier enabling receptors to engage their targets.
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
