产品号 #60015_C
Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e
概述
The 145-2C11 antibody reacts with the ~20 kDa CD3ε subunit of the mouse T cell receptor (TCR)/CD3 complex, which is expressed on the surface of circulating mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severed combined immunodeficiency (SCID). The 145-2C11 antibody has been used for in vitro functional (blocking and activation) assays and has been reported to block binding by the clone 17A2 antibody. The 145-2C11 antibody is not recommended for use with formalin-fixed, paraffin-embedded sections.
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse T Cell Isolation Kit (Catalog #19851) and EasySep™ Mouse CD90.2 Positive Selection Kit II (Catalog #18951).
Subtype
Primary Antibodies
Target Antigen
CD3e
Alternative Names
CD3, CD3epsilon, T3
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Hamster
Cell Type
T Cells
Species
Mouse
Application
Cell Isolation, ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
145-2C11
Gene ID
12501
Isotype
IgG1, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
