产品号 #60001_C
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b
概述
The M1/70 antibody reacts with CD11b, an ~170 kDa type 1 transmembrane glycoprotein which associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen, Mac-1 functions in several processes, including the adherence of neutrophils and monocytes to stimulated endothelium and phagocytosis of complement-coated particles. CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. CD11b is a relatively late marker for myeloid differentiation and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. The M1/70 antibody reportedly blocks iC3b binding to Mac-1. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Isolation Kit (Catalog #19861).
Subtype
Primary Antibodies
Target Antigen
CD11b
Alternative Names
alphaM integrin, C3biR, CR3, Ly-40, Mac-1, Mo1
Reactive Species
Baboon, Chimpanzee, Human, Rabbit, Rhesus
Conjugation
Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
Granulocytes and Subsets, Monocytes
Species
Human, Mouse, Non-Human Primate, Other
Application
Cell Isolation, CyTOF, FACS, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blotting
Area of Interest
Immunology
Clone
M1/70
Gene ID
16409
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
Figure 3. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).
Figure 4. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa APC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 5. Data for Biotin-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa biotin isotype control antibody followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.
Figure 6. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.
Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
Figure 8. Data for PB-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC Catalog #
60001AZ, 60001AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin Catalog #
100-0439, 100-0438, 60001BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC Catalog #
100-0437, 100-0436, 60001FI Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE Catalog #
100-0435, 100-0434 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 Catalog #
60001PS, 60001PS.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70 Catalog #
100-0433 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC Catalog #
60001AZ, 60001AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin Catalog #
100-0439, 100-0438, 60001BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC Catalog #
100-0437, 100-0436, 60001FI Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE Catalog #
100-0435, 100-0434 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 Catalog #
60001PS, 60001PS.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD11b Antibody, Clone M1/70 Catalog #
100-0433 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Granulocyte Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Total Lymphocyte Research
Resources and Publications
Educational Materials (4)
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Legal Statement: Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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