产品号 #60034_C
Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit)
概述
The ACK2 antibody reacts with CD117 (c-Kit), an ~145 kDa type 1 transmembrane receptor for c-Kit ligand (stem cell factor/steel factor) that is broadly expressed on hematopoietic stem cells in bone marrow, including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors, as well as on mast cells. CD117 belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains and a cytoplasmic protein kinase domain. Binding of c-Kit ligand to CD117 induces dimerization and autophosphorylation, which activates several intracellular signaling pathways critical for the proliferation and differentiation of hematopoietic stem cells. Signaling by CD117 is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. Mutations in CD117 are associated with various types of tumors and the piebald trait, an autosomal dominant abnormality of pigmentation. It has been reported that binding of the ACK2 antibody blocks the function of CD117.
Subtype
Primary Antibodies
Target Antigen
CD117 (c-Kit)
Alternative Names
c-KIT, cKIT, Stem cell factor receptor (SCFR)
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Mouse
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Stem Cell Biology
Clone
ACK2
Gene ID
16590
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).
Figure 2. Data for Unconjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (Catalog #60019PE). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE.
Figure 3. Data for AlexaFluor® 488-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, Alexa Fluor® 488 and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (Catalog #60077AD) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.
Figure 4. Data for APC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.
Figure 5. Data for FITC-Conjugated
(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD117 Antibody, Clone ACK2, FITC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, APC Catalog #
60034AZ, 60034AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, PE Catalog #
60034PE, 60034PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Mouse CD117 Antibody, Clone ACK2 Catalog #
60034.1, 60034 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, APC Catalog #
60034AZ, 60034AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD117 Antibody, Clone ACK2, PE Catalog #
60034PE, 60034PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Mouse CD117 Antibody, Clone ACK2 Catalog #
60034.1, 60034 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Resources and Publications
Educational Materials (2)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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