产品号 #100-1063_C
Mouse monoclonal antibody against human E-cadherin (CD324)
概述
Clone 7G5D4 is a mouse monoclonal antibody that reacts with human E-cadherin (CD324), a transmembrane glycoprotein present in the adherens junction of epithelial cells. E-cadherin consists of a large extracellular domain composed of five cadherin-motif subdomains, a single-pass transmembrane segment, and a short conserved cytoplasmic domain. Use this polyclonal antibody in applications requiring detection of E-cadherin. It is verified for use with immunocytochemistry (ICC) and immunofluorescence (IF).
E-cadherin is a calcium dependent cell-cell adhesion protein that plays a central role in the growth and development of cells by controlling tissue architecture and maintaining tissue integrity. It consists of a large extracellular domain composed of five cadherin-motif subdomains, a single-pass transmembrane segment, and a short conserved cytoplasmic domain. As a key regulator of epithelial junction formation, E-cadherin associates with catenins and is necessary for cell-cell adhesion. The E-cadherin-catenin complex is associated with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Disruption of this complex by tyrosine phosphorylation results in changes to cell adhesion properties. The expression of E-cadherin has been suggested in tumor metastasis.
Subtype
Primary Antibodies
Target Antigen
E-Cadherin (CD324)
Alternative Names
Arc-1, CD324, CDH1, CDHE, E-cad, E-Cadherin, ECAD, LCAM, UVO
Reactive Species
Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Intestinal Cells
Species
Human
Application
Immunocytochemistry, Immunofluorescence
Area of Interest
Organoids
Clone
7G5D4
Gene ID
999
Isotype
IgG1
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
