抗人E-钙黏蛋白(CD324)抗体,克隆7G5D4

小鼠单克隆抗体,抗人E-钙黏蛋白(CD324)

产品号 #(选择产品)

产品号 #100-1063_C

小鼠单克隆抗体,抗人E-钙黏蛋白(CD324)

总览

克隆7G5D4是一种小鼠单克隆抗体,可与人E-钙黏蛋白 (CD324)反应。E-钙黏蛋白是一种存在于上皮细胞黏附连接处的跨膜糖蛋白,由五个钙黏蛋白基序亚结构域、一个单次跨膜结构段以及一个短的保守胞质结构域组成的大型胞外结构域构成。该单克隆抗体适用于需要检测E-钙黏蛋白的实验应用,并已验证可用于免疫细胞化学(ICC)和免疫荧光(IF)实验。E-钙黏蛋白是一种钙依赖性细胞黏附蛋白,在调控组织结构和维持组织完整性方面,对细胞的生长和发育发挥着关键作用。它由五个钙黏蛋白基序亚结构域、一个单次跨膜结构段以及一个短的保守胞质结构域组成的大型胞外结构域构成。作为上皮细胞连接形成的关键调控因子,E-钙黏蛋白可与连接蛋白结合,并对细胞间黏附至关重要。E-钙黏蛋白-连接蛋白复合物与皮质肌动蛋白束共同定位于黏附带及侧向黏附斑。已有研究提出,E-钙黏蛋白的表达与肿瘤转移有关。

 

亚型
一抗
 
靶抗原
E-Cadherin (CD324)
 
别名
Arc-1;CD324;CDH1;CDHE;E-cad;E-钙黏附蛋白;ECAD;LCAM;UVO
 
活性物种

 
偶联
未偶联的
 
宿主物种
小鼠
 
细胞类型
肠道细胞
 
种属

 
应用
免疫细胞化学,免疫荧光
 
研究领域
类器官
 
克隆
7G5D4
 
基因编号
999
 
同种型
IgG1
 

Data Figures

Figure 1. Anti-Human E-Cadherin (CD324) Antibody, Clone 7G5D4 is Suitable for Staining Human Intestinal Organoids

H9 human intestinal organoids were cultured using STEMdiff™ Intestinal Organoid Kit (Catalog #05140), then fixed and labeled with Anti-Human E-Cadherin (CD324) Antibody, Clone 7G5D4, followed by Goat Anti-mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (Catalog #100-1069). Nuclei were counter-stained with DAPI (blue). Inset shows cells labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1063
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Clone 7G5D4
Gene Id 999
Alternative Names Arc-1, CD324, CDH1, CDHE, E-cad, E-Cadherin, ECAD, LCAM, UVO
Isotype IgG1
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