产品号 #100-1063_C
小鼠单克隆抗体,抗人E-钙黏蛋白(CD324)
克隆7G5D4是一种小鼠单克隆抗体,可与人E-钙黏蛋白 (CD324)反应。E-钙黏蛋白是一种存在于上皮细胞黏附连接处的跨膜糖蛋白,由五个钙黏蛋白基序亚结构域、一个单次跨膜结构段以及一个短的保守胞质结构域组成的大型胞外结构域构成。该单克隆抗体适用于需要检测E-钙黏蛋白的实验应用,并已验证可用于免疫细胞化学(ICC)和免疫荧光(IF)实验。E-钙黏蛋白是一种钙依赖性细胞黏附蛋白,在调控组织结构和维持组织完整性方面,对细胞的生长和发育发挥着关键作用。它由五个钙黏蛋白基序亚结构域、一个单次跨膜结构段以及一个短的保守胞质结构域组成的大型胞外结构域构成。作为上皮细胞连接形成的关键调控因子,E-钙黏蛋白可与连接蛋白结合,并对细胞间黏附至关重要。E-钙黏蛋白-连接蛋白复合物与皮质肌动蛋白束共同定位于黏附带及侧向黏附斑。已有研究提出,E-钙黏蛋白的表达与肿瘤转移有关。
亚型
一抗
靶抗原
E-Cadherin (CD324)
别名
Arc-1;CD324;CDH1;CDHE;E-cad;E-钙黏附蛋白;ECAD;LCAM;UVO
活性物种
人
偶联
未偶联的
宿主物种
小鼠
细胞类型
肠道细胞
种属
人
应用
免疫细胞化学,免疫荧光
研究领域
类器官
克隆
7G5D4
基因编号
999
同种型
IgG1
Figure 1. Anti-Human E-Cadherin (CD324) Antibody, Clone 7G5D4 is Suitable for Staining Human Intestinal Organoids
H9 human intestinal organoids were cultured using STEMdiff™ Intestinal Organoid Kit (Catalog #05140), then fixed and labeled with Anti-Human E-Cadherin (CD324) Antibody, Clone 7G5D4, followed by Goat Anti-mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (Catalog #100-1069). Nuclei were counter-stained with DAPI (blue). Inset shows cells labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining).
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Species | Human |
---|---|
Clone | 7G5D4 |
Gene Id | 999 |
Alternative Names | Arc-1, CD324, CDH1, CDHE, E-cad, E-Cadherin, ECAD, LCAM, UVO |
Isotype | IgG1 |
DNA标记染料
扫描二维码或搜索微信号STEMCELLTech,即可关注我们的微信平台,第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题,欢迎发消息给STEMCELLTech微信公众平台,或与我们通过电话/邮件联系:400 885 9050 INFO.CN@STEMCELL.COM。