产品号 #60107_C
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83
概述
The HB15e antibody reacts with CD83, an ~45 kDa type I transmembrane glycoprotein and member of the Siglec protein family, containing an extracellular V-type Ig-like domain and a C-terminal cytoplasmic tail. A soluble form of CD83 has also been identified. CD83 is expressed predominantly on circulating and tissue dendritic cells, including Langerhan cells and interdigitating reticulum cells. It is expressed weakly on activated lymphocytes and may also be found on neutrophils, monocytes and macrophages. Expression of CD83 is typically transient, except on dendritic cells, and correlates with upregulation of HLA class II antigen. Its function, though ill-defined, is essential for the development and maintenance of the immune system and evidence suggests it may be involved in cell adhesion and signaling, antigen presentation, and lymphocyte activation. The soluble form of CD83 appears to be immunosuppressive. The epitope on CD83 bound by the HB15e antibody reportedly overlaps with that of antibody 1E11.
Subtype
Primary Antibodies
Target Antigen
CD83
Alternative Names
B-cell activation protein, BL11, HB15
Reactive Species
Baboon, Chimpanzee, Cynomolgus, Human, Macaque, Rhesus
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Dendritic Cells
Species
Human, Non-Human Primate
Application
Flow Cytometry, Immunoblotting, Immunohistochemistry
Area of Interest
Immunology
Clone
HB15e
Gene ID
9308
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).
Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 (solid line histogram).
Figure 3. Data for APC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).
Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).
Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).
Figure 6. Data for PE-Conjugated
Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD83 Antibody, Clone HB15e, APC Catalog #
60107AZ, 60107AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD83 Antibody, Clone HB15e, FITC Catalog #
60107FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD83 Antibody, Clone HB15e, PE Catalog #
60107PE, 60107PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD83 Antibody, Clone HB15e, APC Catalog #
60107AZ, 60107AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD83 Antibody, Clone HB15e, FITC Catalog #
60107FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD83 Antibody, Clone HB15e, PE Catalog #
60107PE, 60107PE.1 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Total Lymphocyte Research
Resources and Publications
Educational Materials (2)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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